FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313983
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60660.46164980825474905No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56830.43250179035801833No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41300.31431152457832406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41280.314159315607584No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36890.2807494465301302No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36640.27884683439587876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35980.2738239383614552No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35900.2732151024784948No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG35050.26674622122204017No Hit
GTGTAGGGGATGTTGACACTCTGCTGACAGTAGTAAGTTGCAACATCTTC33320.25358014525302075No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32700.2488616671600774No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32030.2437626666402838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31490.23965302443030084No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG30710.23371687457143664No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATGTTGCAACTTACTACTGTCAGCAGAGTGTCAACATC30430.2315859489810751No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29860.227247993314982No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28770.21895260440964612No Hit
CCCCTACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG28360.21583232050947387No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28210.21469075322892306No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27680.21065721550431019No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27520.20943954373838933No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27470.20905902131153906No Hit
GTGTCAACATCCCCTACACTTTCGGCCCTGGGACCAAAGTGGATATCAAA26680.20304676696730478No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC26540.20198130417212398No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25680.19543631843029932No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG25460.19376201975215812No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25100.19102225827883618No Hit
GATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGAGACAGAGTTCA24250.18455337702238156No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24220.1843250635662714No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23780.18097646620998903No Hit
CCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATGATGTCTG23560.17930216753184783No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC22510.17131119656799212No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22400.17047404722892154No Hit
GAGTGAACTCTGTCTCAGATCCACTGCCACTGAATCTTGATGGGACCCCA21870.16644050950430866No Hit
ACTTACTACTGTCAGCAGAGTGTCAACATCCCCTACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA20990.1597433147917439No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20890.15898226993804335No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20710.15761238920138237No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20600.1567752398623118No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20460.15570977706713102No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20080.15281780662306893No Hit
ATCTGAGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATG19840.15099129897418764No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19330.14710997022031488No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19290.14680555227883466No Hit
GTCCTGATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT18560.1412499248468207No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18450.1404127755077501No Hit
GTAGTAAGTTGCAACATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCTCAGATCCACTGCCA18360.1397278351394196No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17870.13599871535628694No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17800.13546598395869658No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC17700.13470493910499604No Hit
GTCTCAGATCCACTGCCACTGAATCTTGATGGGACCCCACTTTGCAAACT17540.13348726733907515No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT17530.1334111628537051No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17180.1307475058657532No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16970.12914931167298208No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16900.1286165802753917No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16630.12656175917040025No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16610.12640955019966013No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16510.1256485053459596No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16480.1254201918898494No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16360.12450693806540876No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16350.1244308335800387No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16210.12336537078485793No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16040.12207159453356703No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16020.12191938556282692No Hit
CAGTAGTAAGTTGCAACATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCTCAGATCCACTGCCA15910.12108223622375631No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15590.11864689269191458No Hit
CCCCTAAGGTCCTGATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCA15550.11834247475043436No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15540.11826637026506433No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15450.11758142989673381No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15390.1171248029845135No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15350.11682038504303327No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15340.11674428055766321No Hit
GCCCCTAAGGTCCTGATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGAT15280.1162876536454429No Hit
CATCAAGATTCAGTGGCAGTGGATCTGAGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATGTTG15020.11430893702582148No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15000.11415672805508137No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14980.11400451908434128No Hit
AATCAGGACCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATGATGTCTGCTAATGCTCTGACTTGC14980.11400451908434128No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14860.11309126525990063No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14820.1127868473184204No Hit
GTCTCCTACAGATGCAGACAGGGAGGACGGAGACTGGGTCATCTGGATGT14750.11225411592083002No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14560.11080813069879898No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14340.10913383202065781No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14260.10852499613769737No Hit
ATCTTGATGGGACCCCACTTTGCAAACTGGATGTAGCATAAATCAGGACCTTAGGGGCTTTCCCGGGTTTCTG14100.10730732437177651No Hit
GCATAAATCAGGACCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATGATGTCTGCTAATGCTCTGA14040.10685069745955617No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA14010.10662238400344602No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13990.10647017503270591No Hit
GTCTGCAACCTGAAGATGTTGCAACTTACTACTGTCAGCAGAGTGTCAACATCCCCTACACTTTCGGCCCTGGGA13940.10608965260585562No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13770.10479587635456472No Hit
CACCATCAGCAGTCTGCAACCTGAAGATGTTGCAACTTACTACTGTCAGCAGAGTGTCAACATCCCCTACACTTT13500.10274105524957323No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13490.10266495076420318No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13400.1019800103958727No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13210.10053402517384168No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13210.10053402517384168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)