FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313983
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59190.45046244890535114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49680.37808708331842955No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44540.3389693778382216No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42790.32565109289846217No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37850.2880554771256553No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36280.2761070729225568No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34120.25966850408262515No Hit
GTGTAGGGGATGTTGACACTCTGCTGACAGTAGTAAGTTGCAACATCTTC32840.2499271299552582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32660.24855724921859718No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31730.24147953207918216No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATGTTGCAACTTACTACTGTCAGCAGAGTGTCAACATC29890.22747630677109215No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29680.22587811257832102No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29620.2254214856661007No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG29170.22199678382444826No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28630.21788714161446532No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28600.2176588281583552No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28430.21636505190706423No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27750.21118994690190054No Hit
GTGTCAACATCCCCTACACTTTCGGCCCTGGGACCAAAGTGGATATCAAA27260.2074608271187679No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27180.20685199123580744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26960.20517769255766627No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG26390.20083973689157317No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26330.20038310997935288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26250.19977427409639242No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26030.19809997541825122No Hit
CCCCTACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG25140.1913266762203164No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC24800.18873912371773455No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24670.18774976540792385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24340.18523831739071206No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23610.1796826899586981No Hit
GAGTGAACTCTGTCTCAGATCCACTGCCACTGAATCTTGATGGGACCCCA23420.17823670473666706No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23380.17793228679518686No Hit
GATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGAGACAGAGTTCA22420.17062625619966165No Hit
CCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATGATGTCTG22260.16940858443374077No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22070.16796259921170975No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC21610.16446179288468724No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20400.15525315015491067No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19700.1499258361790069No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19500.1484037464716058No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18960.14429410426162287No Hit
ACTTACTACTGTCAGCAGAGTGTCAACATCCCCTACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA18870.14360916389329237No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18690.1422392831566314No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18620.14170655175904104No Hit
ATCTGAGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATG18530.14102161139071054No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18460.14048887999312015No Hit
GTCCTGATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT18440.14033667102238004No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18390.1399561485955298No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC18380.13988004411015972No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18210.1385862678588688No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18120.1379013274905383No Hit
GTAGTAAGTTGCAACATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCTCAGATCCACTGCCA17800.13546598395869658No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17210.13097581932186336No Hit
GTCTCAGATCCACTGCCACTGAATCTTGATGGGACCCCACTTTGCAAACT17160.1305952968950131No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16990.12930152064372216No Hit
GTGGGGTCCCATCAAGATTCAGTGGCAGTGGATCTGAGACAGAGTTCACT16990.12930152064372216No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16920.1287687892461318No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16670.12686617711188045No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16150.12290874387263762No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15800.12024508688468571No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15770.12001677342857554No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15740.1197884599724654No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15670.11925572857487501No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15660.11917962408950496No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15080.11476556393804183No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14890.11331957871601077No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14890.11331957871601077No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14740.11217801143545997No Hit
AATCAGGACCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATGATGTCTGCTAATGCTCTGACTTGC14550.11073202621342895No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14540.1106559217280589No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14400.10959045893287812No Hit
CCCCTAAGGTCCTGATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCA14310.10890551856454764No Hit
CAGTAGTAAGTTGCAACATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCTCAGATCCACTGCCA14310.10890551856454764No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14110.10738342885714657No Hit
GCCCCTAAGGTCCTGATTTATGCTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGCAGTGGAT13880.10563302569363531No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13850.10540471223752515No Hit
GTCTCCTACAGATGCAGACAGGGAGGACGGAGACTGGGTCATCTGGATGT13820.10517639878141498No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13720.10441535392771444No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13700.10426314495697432No Hit
GCATAAATCAGGACCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAATTTAAATGATGTCTGCTAATGCTCTGA13670.10403483150086418No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13620.10365430907401389No Hit
CATCAAGATTCAGTGGCAGTGGATCTGAGACAGAGTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATGTTG13580.10334989113253366No Hit
ATCTTGATGGGACCCCACTTTGCAAACTGGATGTAGCATAAATCAGGACCTTAGGGGCTTTCCCGGGTTTCTG13540.10304547319105346No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13330.10144727899828233No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13220.10061012965921173No Hit
CTGCTGATGGTGAGAGTGAACTCTGTCTCAGATCCACTGCCACTGAATCT13220.10061012965921173No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13160.10015350274699139No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13160.10015350274699139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2400.042.429841
GTCGTAA451.0740809E-437.8814130
CGTAAAC501.9771188E-434.14815532
GTATCAA18500.034.127351
CGGTGTA400.002800274633.9723524
ATAGGAT552.4251857E-432.98061863
GACAGGT1550.032.8489071
TTAGAGC450.00502029530.1539484
TCCGAGA6250.028.91737670
TATCAAC21700.028.7709392
GGTCGTA605.8343593E-428.39258429
TGTTAGA16900.028.26349670
ATCAACG22200.027.9615823
TCAACGC22550.027.5296864
AGGTACG500.00838913727.1447542
CAACGCA23250.026.8498085
AACGCAG23700.026.767426
TCGTAAA659.237022E-426.2456831
AATGGTA5050.025.5634570
CGCAGAG25300.024.9414798
Produced by FastQC (version 0.11.5)