FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185644
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT89440.4092157734745457No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81550.3731165734218381No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70760.32374897284278686No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCA68630.3140035614217137No Hit
GTTGTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGT58800.2690282589479348No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51170.23411863963207183No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49960.22858251389521805No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47520.21741875621098403No Hit
CCCTTAGACTCTCCTGCGCAGCCTCTGGACTCACCTTTAGTATCGCCTGG45790.2095034690004411No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACT45660.20890867863201878No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45210.20684978889517233No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT44730.20465363984253612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41820.19133948621092914No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCTGGGGAGAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG41270.18882306542145014No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40950.18735896605302602No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCAGTGGCCCTGGT40910.18717595363197298No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38970.1782998512109017No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38660.1768815049477408No Hit
GGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCTGG37910.17345002205299673No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37890.17335851584247022No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA36920.16892046463193455No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36860.16864594600035504No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC35920.16434515410560915No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35920.16434515410560915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35700.16333858578981755No Hit
GTGTATTGTCTCACCGTTAGCTTTGCTCTTAATAAGGGCAACCCACTCCAGCCCCTTACCTGGAGCCTGGCGGAC35430.1621032519477097No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC35370.16182873331613015No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35340.1616914740003404No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC34880.15958683115823072No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT33650.15395919921085044No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA33280.15226633431611003No Hit
GTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA33270.15222058121084678No Hit
CCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGTAAGGGG33030.15112250668452867No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32220.1474165051582051No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTT32150.1470962334213623No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG32020.14650144305294No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT31740.14522035610556888No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31100.1422921573687206No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30990.1417888732108248No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC30900.14137709526345554No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC30240.13835739031608077No Hit
GTCTAAGGGACTCCCCAGGCTTTACCAAGGTTCCCCCAGACTCCACAAGT30000.13725931578976264No Hit
CCATAGATGAGGAGTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGC29660.135703710210812No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGG28890.13218072110554144No Hit
CTCCCGGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA28800.13176894315817214No Hit
GACAATACACTACGCAGCACCCGTGAAAGACAGATTCACCATCTCAAGAGATGATTCAGAAAACAGTGTCTAT28630.13099114036869683No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC28620.13094538726343355No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA28570.1307166217371173No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28180.1289322506318504No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCA27690.12669034847395091No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA27630.1264158298423714No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27550.12604980500026536No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27510.12586679257921235No Hit
CTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT27180.12435694010552498No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT26850.12284708763183756No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26320.1204221730528851No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25990.1189123205791977No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGG25880.11840903642130192No Hit
CTGCTACTCTGGCTCCCAGATGGCACTGGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTC25390.11616713426340246No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC25320.11584686252655968No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC25280.11566385010550666No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25130.11497755352655784No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24680.11291866378971141No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG24310.111225798894971No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24030.10994471194759989No Hit
GCCTCTGGACTCACCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGC23820.10898389673707155No Hit
GTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGCTAAATTGCTGCTG23700.10843485947391249No Hit
TTGTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTC23390.10701651321075162No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23170.10600994489496003No Hit
GTCAGAGTGTCAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACTCCTCATCTATGGTG23080.10559816694759074No Hit
GTCTGCGTCTCCTGGGGAGAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAATTTAGCCTGGTA22920.10486611726337866No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT22840.10450009242127262No Hit
TTATTGTACCACATGGGGATACAACTTTTTTACCTTTGACTATTGGGGCC22750.10408831447390335No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22620.10349352410548103No Hit
CCTTGGGGCTGGTCGGGGATGCCGTGGAGACGGTGACCAGGGCTCCCTGG22430.1026242151054792No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22300.10202942473705691No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22140.10129737505284483No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC22130.10125162194758158No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG21990.10061107847389601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1700.048.79413641
TCGTATG1700.042.62940240
GGTATCA15100.042.3311271
ACCGATC3300.042.2801727
GACCGAT3450.040.4298626
TATCTCG2600.039.57135436
ACGAGAC3700.038.58487722
GTATCAA41350.037.9879071
CCGATCA3750.037.22872528
ATCTCCG3950.036.99064310
CCCACGA4050.035.2463519
GCCGTCT2100.034.81282446
TATCAAC45300.034.2813872
ATCAACG46050.033.944443
CACGAGA4250.033.59077521
GTGGTAT4150.033.5903631
TCAACGC46500.033.5428624
CGATCAG4200.033.26433629
CAACGCA47250.033.0823555
ATCTCGT2700.033.06238637
Produced by FastQC (version 0.11.5)