FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185644
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT84570.3869340112113409No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81800.3742604010534195No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCA63590.29094399636903356No Hit
GTTGTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGT60600.27726381789532056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59890.27401534742162953No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53130.24308624826366965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49720.22748443936889995No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48050.21984367078993652No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATT46800.21412453263202977No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46650.21343823605308093No Hit
CCCTTAGACTCTCCTGCGCAGCCTCTGGACTCACCTTTAGTATCGCCTGG45090.20630075163201328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43700.1999410700004209No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACT42860.19609780915830757No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39550.18095353131617045No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCAGTGGCCCTGGT38820.17761355463195289No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCTGGGGAGAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG37370.170979354368781No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC36830.16850868668456528No Hit
GTGTATTGTCTCACCGTTAGCTTTGCTCTTAATAAGGGCAACCCACTCCAGCCCCTTACCTGGAGCCTGGCGGAC36690.16786814321087973No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36360.16635829073719233No Hit
GGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCTGG35670.1632013264740278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35080.1605018932634958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35010.16018162152665302No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC34760.15903779389507167No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34730.15890053457928188No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC34240.15665863242138245No Hit
GTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA33460.15308989021084862No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC33440.1529983840003221No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33170.15176305015821423No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32700.14961265421084127No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32700.14961265421084127No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32660.14942964178978826No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32260.14759951757925813No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT32080.14677596168451953No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31650.14480857815819959No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGG31110.14233791047398386No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30700.14046203315819045No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA30670.1403247738424007No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT30490.1395012179476621No Hit
CCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGTAAGGGG30460.13936395863187234No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30220.13826588410555424No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG29990.13721356268449938No Hit
CACCATCACCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAACAACTGGCCTCGGACGTT29980.13716780957923613No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC28650.13108264657922333No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28580.13076237484238054No Hit
CTCCCGGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA28560.13067086863185404No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATACCA28100.12856622578974436No Hit
GTCTAAGGGACTCCCCAGGCTTTACCAAGGTTCCCCCAGACTCCACAAGT27560.1260955581055286No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27400.12536350842131658No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27220.12453995252657797No Hit
CCATAGATGAGGAGTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGC27210.12449419942131472No Hit
GACAATACACTACGCAGCACCCGTGAAAGACAGATTCACCATCTCAAGAGATGATTCAGAAAACAGTGTCTAT27130.12412817457920869No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26750.12238955657920504No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA26680.12206928484236226No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26540.12142874136867671No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA26480.12115422273709715No Hit
CTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT26160.11969012336867303No Hit
CTGCTACTCTGGCTCCCAGATGGCACTGGAGAAACAGTGATGACGCAGTCTCCAGCCACCCTGTCTGCGTCTC25520.11676192463182476No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC25240.11548083768445365No Hit
GTCCGGGAGCCTGGCCAGGTTTCTGCCGGTACCAGGCTAAATTGCTGCTG24960.11419975073708254No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24820.11355920726339698No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23990.10976169952654687No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGG23900.10934992157917758No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23840.10907540294759804No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT23650.10820609394759623No Hit
TTGTTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTC23200.10614720421074977No Hit
GCCTCTGGACTCACCTTTAGTATCGCCTGGATGAGCTGGGTCCGCCAGGC22980.10514063589495819No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22930.10491187036864191No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC22880.10468310484232565No Hit
GCCTTGGGATTCTCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA22640.10358503031600755No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22560.1032190054739015No Hit
GTCTGCGTCTCCTGGGGAGAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCAATTTAGCCTGGTA22470.10280722752653224No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22440.10266996821074247No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22220.10166339989495086No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22190.1015261405791611No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG22140.10129737505284483No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG22030.10079409089494905No Hit
GTCAGAGTGTCAGCAGCAATTTAGCCTGGTACCGGCAGAAACCTGGCCAGGCTCCCGGACTCCTCATCTATGGTG22020.10074833778968578No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22020.10074833778968578No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21910.10024505363178998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT252.7416577E-454.47223728
CGTAATT607.12997E-444.87528670
TAACGTA359.118496E-442.69013267
GGTATCA15900.039.952461
ACGTAAT450.001396751539.14459669
GTATCAA39700.036.3661651
TATCAAC43800.032.86792
ATCAACG45650.031.5987053
AGCGTTA450.004132651731.378354
TCAACGC46550.030.9863574
CAACGCA46650.030.9206455
AACGCAG47200.030.4877346
CGCAGAG49200.029.3863588
AGAGTAC49300.029.05204811
GCAGAGT50500.028.5620179
TACGTAT950.00679497328.34228570
GTGGTAT4950.027.450731
TAACCGT1056.3801235E-726.85349553
TGTTAGA18700.026.637270
ACGAACT30150.025.32446152
Produced by FastQC (version 0.11.5)