FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093232
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGCTCATTTCCGAGGACCTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAA38970.3564659651382323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31900.29179533712880706No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29640.271122689419995No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26120.2389245832540577No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA25450.23279596645542758No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT25270.23114947239012396No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24090.2203557890731336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23250.21267215010171672No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22250.20352496085003No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21920.20050638839697338No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20120.18404144774393724No Hit
GTGTATTACTGTCAGCAATATGGTAGCTCATTTCCGAGGACCTTCGGCCA19360.1770895839126553No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18740.17141832657660952No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18260.1670276757357999No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18170.16620442870314808No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17370.1588866773017987No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT17330.15852078973173123No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16910.1546789702460228No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA16660.15239217293310112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16390.1499224318351457No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16260.14873329723242643No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16050.1468123874895722No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15120.13830550148550355No Hit
GAGTATTACCAAAGACTACTTAGCCTGGTACCAGCAGAAACCCGGCCAGGCTCCCAGGCTCCTCGTCTATGGTGT14530.13290865982700836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14230.13016450305150234No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14180.129707143588918No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC13950.12760329006103005No Hit
CGTCTATGGTGTCTCCACCAGGGCCACTGGCGTCCCTGACAGATTCAGTGGCAGTGGGTCTGGCACAGACTTCA13720.12549943653314208No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13260.12129172947736619No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA12840.11744990999165777No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12750.11662666295900595No Hit
GTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCCCTTGGCCGAAGGTCCTCGGAAATGAGCTACCATATTG12750.11662666295900595No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA12530.11461428132363487No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12480.11415692186105054No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12350.11296778725833126No Hit
GTAATAAACAGCCGTGTCCTCGACTCTCAGGCTATTCATTTCCAGATGCA12110.11077246183792645No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA12070.11040657426785896No Hit
CTCCTGCAGGGCTAGTCAGAGTATTACCAAAGACTACTTAGCCTGGTACC12070.11040657426785896No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11610.10619886721208308No Hit
GTATCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCT11610.10619886721208308No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11540.10555856396446499No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11460.10482678882433007No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11410.10436942936174572No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11380.10409501368419513No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11330.10363765422161078No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11300.10336323854406018No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCC10980.10043613798352041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG209.758501E-567.1246526
GGTATCA13550.045.2302251
CGTATGC4150.043.2071941
TCGTATG4200.042.5864740
CTACGGC405.9214934E-541.90870325
GTCGTAT1350.040.65571239
GCCGTCT4550.040.00437546
ATCTCGT2900.039.97962637
ATGCCGT4700.039.24714744
CGATGTC1650.039.10481635
GAGCGAT4500.039.04995732
CCCACGA4750.038.77273619
GTATCAA37500.038.4913331
CTTATAC5100.038.0868231
CCGTCTT4950.037.579547
ATCTCCG4900.037.5720610
GTGGTAT2950.037.4635431
ACGAGAC4950.037.2214922
ACTACGT451.19518925E-437.199353
CGTCTTC4850.037.02775248
Produced by FastQC (version 0.11.5)