FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672024
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCTTGGGATGACGCCCTGAATAGTTGGATGTTCGG42480.6321202814185207No Hit
GTAATAATCAGCCTCATCGTCAGACCGGAGGCCACTGATGGCCAGGGAGG38580.5740866397628657No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36170.5382248253038582No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26660.3967120221896837No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26310.39150387486161203No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCC25470.3790043212742402No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGCGTCATCCCAAG25260.3758794328773972No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23450.3489458709807983No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21600.32141709224670545No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCAC21520.32022665857171767No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCC21180.3151673154530195No Hit
GTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGT21030.31293525231241737No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20880.3107031891718153No Hit
GCCCCCACACTCCTCATCTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGT20370.3031141744937681No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAACTATTCAGG20310.30222134923752725No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19920.2964179850719617No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19210.285852886206445No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19120.28451364832208376No Hit
CCTATGAACGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18260.2717164863159649No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17920.26665714319726674No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17610.26204421270668904No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17400.25891932430984604No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17370.25847291168172565No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15870.23615228027570445No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15420.22945609085389806No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14950.22246229301334475No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14860.22112305512898348No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14680.21844457936026096No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14340.21338523624156283No Hit
GTGTGGGGGCCGTGCCTGGCAACTGACGATACCAGGAGACATAATTATAT14320.21308762782281587No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14180.21100436889158722No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT14170.21085556468221373No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14030.20877230575098507No Hit
GAATAGTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13810.20549861314476864No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTC13780.20505220051664821No Hit
CTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC13690.20371296263228694No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGC13270.197463185838601No Hit
CTCCTCATCTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATT12570.18704689118245776No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12550.18674928276371083No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12250.18228515648250657No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12030.17901146387629013No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG11630.17305929550135113No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11490.1709760365701225No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11420.16993440710450816No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11220.1669583229170387No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTCCTCACTCACTGTGCAGGGTCCTGGGC11100.165172672404557No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11090.1650238681951835No Hit
AGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGG10990.16353582610144876No Hit
GCTTGGGATGACGCCCTGAATAGTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10900.1621965882170875No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10590.15758365772650976No Hit
ATCCCAAGCTGCACAGTAATAATCAGCCTCATCGTCAGACCGGAGGCCAC9940.1479113841172339No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9870.14686975465161958No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9840.14642334202349916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9820.14612573360475223No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGG9500.141363998904801No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAACGTTCATAGGTTCTCATCCCTCACCCCCCAC9480.14106639048605407No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT9270.13794150208921108No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9260.13779269787983764No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9240.13749508946109068No Hit
CTACAGAACGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACT9100.13541183052986203No Hit
CCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGTCATCTCTTGTTCTGG9100.13541183052986203No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9050.13466780948299467No Hit
GATGAGGAGTGTGGGGGCCGTGCCTGGCAACTGACGATACCAGGAGACAT8940.13303096317988644No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8880.13213813792364557No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT8850.13169172529552514No Hit
GGCCATCAGTGGCCTCCGGTCTGACGATGAGGCTGATTATTACTGTGCAG8840.1315429210861517No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA8820.13124531266740475No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAA8740.13005487899241694No Hit
GTCCTATAGATGAGGAGTGTGGGGGCCGTGCCTGGCAACTGACGATACCA8690.12931085794554956No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8630.12841803268930874No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG8400.12499553587371881No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG8360.1244003190362249No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8360.1244003190362249No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8350.12425151482685143No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8350.12425151482685143No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCTGACGATG8290.12335868957061057No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8190.12187064747687583No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAACGTTCATAGGTTCTCATCCCTCACCCCCCACCA8170.12157303905812888No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8060.11993619275502065No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC7940.11815054224253896No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC7890.11740652119567158No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC7890.11740652119567158No Hit
CTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTC7860.11696010856755115No Hit
CGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT7790.11591847910193684No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7600.1130911991238408No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT7580.11279359070509387No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7500.11160315703010606No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7480.11130554861135912No Hit
GCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGCGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCGT7390.10996631072699783No Hit
GATGAGAACCTATGAACGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7370.1096687023082509No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7350.10937109388950396No Hit
CAACTATTCAGGGCGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATC7220.10743663916764877No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG7220.10743663916764877No Hit
GAGTACGGGAGCTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT7220.10743663916764877No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7180.10684142233015488No Hit
GGAGAAGACAGTGGCCCCTACAGAACGTTCATAGGTTCTCATCCCTCACC7150.10639500970203446No Hit
TCCCTCTCCTCCTCGCCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAG7050.1049069676082997No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGCCACTGTCTTCT7000.10416294656143232No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA6910.10282370867707107No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6910.10282370867707107No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC6900.10267490446769759No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGT6790.10103805816458936No Hit
GGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAG6780.10088925395521588No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG6760.10059164553646895No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC6750.10044284132709547No Hit
CCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT6750.10044284132709547No Hit
CCTCATCTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGC6740.100294037117722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA150.00274771966.36809529
AGGGGGG2700.057.85420670
GACTTAC200.00794470250.75475736
GTATGCC2100.049.6639142
CGTATGC2100.049.43851541
GGGTAGT200.0088427849.39802623
TCGTATG2200.048.4911740
ATGCCGT2100.048.4502244
TCTCGTA2000.046.05275338
GCCAGAG12950.043.8627970
CGAATCT2150.043.7581634
TATGCCG2400.043.66529543
GCCGTCT2350.043.64563446
GGCGAAT2400.043.12807532
CCCACGA2450.043.00044319
GTGGTAT3700.042.7130781
TATACAC2650.042.2241023
TAAGGCG2600.042.11821429
ACTAAGG2600.041.9670727
GACTAAG2600.041.91067526
Produced by FastQC (version 0.11.5)