FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672024
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCTTGGGATGACGCCCTGAATAGTTGGATGTTCGG39690.5906039070033213No Hit
GTAATAATCAGCCTCATCGTCAGACCGGAGGCCACTGATGGCCAGGGAGG37950.5647119745723367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35310.5254276632977394No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33110.4926907372355749No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26350.39209909169910595No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25990.3867421401616609No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCC25610.38108758020546885No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24610.36620715926812136No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGCGTCATCCCAAG24520.3648679213837601No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23920.35593966882135164No Hit
GTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGT21900.3258812185279097No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCACCCTCAGCGTCC20760.3089175386593336No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20160.2999892860969251No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAACCAC19840.2952275513969739No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19230.286150494625192No Hit
GCCCCCACACTCCTCATCTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGT18540.27588300417842215No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18100.26933561896598934No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18050.26859159791912196No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17810.2650202968941585No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAACTATTCAGG17800.26487149268478505No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17540.2610025832410747No Hit
GTGTGGGGGCCGTGCCTGGCAACTGACGATACCAGGAGACATAATTATAT17220.25624084854112356No Hit
CCTATGAACGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16760.2493958549099437No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15170.22573598561956124No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15160.22558718141018774No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15160.22558718141018774No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14900.22171827196647736No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14520.21606371201028535No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14510.21591490780091185No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14100.2098139352165994No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13940.20743306786662383No Hit
GAATAGTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13900.20683785102912994No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13860.20624263419163602No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12880.1916598216730355No Hit
CTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC12690.18883254169493946No Hit
CTCCTCATCTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATT12680.188683737485566No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTC12620.18779091222932515No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGC12070.17960668071378402No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11770.17514255443257978No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11480.17082723236074904No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11200.16666071449829173No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10900.1621965882170875No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10850.1614525671702201No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10600.15773246193588325No Hit
GTCCTATAGATGAGGAGTGTGGGGGCCGTGCCTGGCAACTGACGATACCA10480.15594681142340155No Hit
AGTCTGTCCTGACTCAACCACCCTCAGCGTCCGGGACCCCCGGGCAGAGG10460.1556492030046546No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10360.15416116091091983No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10250.15252431460781163No Hit
GCTTGGGATGACGCCCTGAATAGTTGGATGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG10230.15222670618906467No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10040.14939942621096866No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9960.14820899253598085No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9910.14746497148911347No Hit
ATCCCAAGCTGCACAGTAATAATCAGCCTCATCGTCAGACCGGAGGCCAC9850.14657214623287265No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9610.14300084520790923No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9360.13928073997357238No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC9350.13913193576419888No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9210.13704867683297026No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9070.1349654179017416No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAACGTTCATAGGTTCTCATCCCTCACCCCCCAC8830.1313941168767782No Hit
GATGAGGAGTGTGGGGGCCGTGCCTGGCAACTGACGATACCAGGAGACAT8760.1303524874111639No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA8660.12886444531742913No Hit
GGCCATCAGTGGCCTCCGGTCTGACGATGAGGCTGATTATTACTGTGCAG8590.12782281585181482No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAA8590.12782281585181482No Hit
CCCTCAGCGTCCGGGACCCCCGGGCAGAGGGTCGTCATCTCTTGTTCTGG8580.12767401164244135No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGG8570.12752520743306786No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC8410.12514434008309228No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8340.12410271061747795No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCTGACGATG8280.12320988536123709No Hit
GGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAG8170.12157303905812888No Hit
CTACAGAACGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACT8090.12038260538314108No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8080.1202338011737676No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT8060.11993619275502065No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8010.11919217170815329No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTCCTCACTCACTGTGCAGGGTCCTGGGC7930.11800173803316548No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7920.11785293382379201No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC7760.11547206647381641No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7750.11532326226444294No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT7720.11487684963632251No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7620.11338880754258776No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC7530.11204956965822649No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7470.11115674440198564No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7460.11100794019261216No Hit
CGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT7430.11056152756449174No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7410.1102639191457448No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG7330.109073485470757No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA7320.10892468126138352No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7300.10862707284263658No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAACGTTCATAGGTTCTCATCCCTCACCCCCCACCA7220.10743663916764877No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT7220.10743663916764877No Hit
GCTTGGTCCCTCCGCCGAACATCCAACTATTCAGGGCGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATCGT7190.10699022653952835No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACGTTCTGTAGGGGCCACTGTCTTCT7120.10594859707391403No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG7080.10535338023642013No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7020.10446055498017927No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7010.1043117507708058No Hit
CAACTATTCAGGGCGTCATCCCAAGCTGCACAGTAATAATCAGCCTCATC6900.10267490446769759No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6840.10178207921145672No Hit
CCTCATCTATAGGACTACTGAACGGACGTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGC6840.10178207921145672No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG6830.10163327500208326No Hit
CTTCAGGTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCGCCCTC6790.10103805816458936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)