EGAF00004692077 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004692077
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13668
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	5976	43.7225636523266	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	651	4.762949956101844	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	375	2.7436347673397714	RNA PCR Primer, Index 39 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	373	2.729002048580626	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	190	1.3901082821188178	RNA PCR Primer, Index 39 (96% over 25bp)
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC	105	0.7682177348551361	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCCGT	104	0.7609013754755634	No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG	98	0.717003219198127	No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTA	87	0.636523266022827	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGT	84	0.6145741878841089	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATATCGTATGCCGT	69	0.504828797190518	No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACA	63	0.46093064091308167	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	58	0.42434884401521805	RNA PCR Primer, Index 39 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	56	0.4097161252560726	No Hit
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCT	43	0.31460345332162715	No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	40	0.292654375182909	No Hit
GCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	34	0.24875621890547264	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	31	0.22680714076675446	RNA PCR Primer, Index 39 (96% over 30bp)
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG	28	0.2048580626280363	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACA	26	0.19022534386889084	No Hit
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC	24	0.17559262510974538	No Hit
GAGACAGTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG	22	0.16095990635059995	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG	21	0.15364354697102722	Illumina PCR Primer Index 11 (96% over 25bp)
CAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC	20	0.1463271875914545	No Hit
GATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG	17	0.12437810945273632	RNA PCR Primer, Index 39 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACCTCTCGTATGCCGT	17	0.12437810945273632	No Hit
CATATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	17	0.12437810945273632	No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	17	0.12437810945273632	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG	14	0.10242903131401815	RNA PCR Primer, Index 39 (96% over 30bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGGGG	115	0.0	74.07965	70
AGGGGGG	505	0.0	67.478485	70
GTGGGGG	20	0.003109002	63.893692	70
GCTCGTA	30	8.028175E-6	58.208813	38
AGCTCGT	30	8.214021E-6	57.986645	37
TACAGCT	30	8.5964275E-6	57.547348	34
AAGGGGG	535	0.0	57.531982	69
CTACAGC	30	8.793095E-6	57.330193	33
TCTACAG	30	8.993478E-6	57.114662	32
AAAATAG	20	0.0049109426	56.971878	65
GTCGTAT	25	2.3286877E-4	55.880455	39
TGTCGTA	25	2.3286877E-4	55.880455	38
ATGTCGT	25	2.3734513E-4	55.66717	37
CATGTCG	25	2.3734513E-4	55.66717	36
ACATGTC	25	2.4188963E-4	55.455513	35
TACATGT	25	2.4650307E-4	55.245457	34
CTACATG	25	2.511862E-4	55.036976	33
GTGTATA	25	2.912544E-4	53.424175	1
GTATAAG	25	2.912544E-4	53.424175	3
TGTATAA	25	2.912544E-4	53.424175	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content