FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625812
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18880.3016880468894812No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16410.26221932465341025No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15150.24208548254108261No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14940.238729842189028No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13000.20773011703195207No Hit
GTGTATTACTGTGCGAGAGAGGGAAAAACCACCAGTGACTACTGGGGCCA11690.18679731293104No Hit
GTGATGGAGACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTT11600.18535918135158802No Hit
GTTTATTACTGCATGCAAGCTACACATTTTCCTCATCGGACGTTCGGCCA10710.17113765795478514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10260.16394700005752527No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10010.1599521901146031No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9870.1577150965465667No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9640.15403987139907832No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAGTCTGTCCCTGCCCCACTGCCA9300.1486069298767042No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA9180.14668942110410155No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9050.144612119933782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8820.14093689478629365No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8790.14045751759314298No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT8450.13502457607076887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8430.13470499127533508No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8210.13118955852556358No Hit
CCCTTGGACAGCCGGCCTCCATCACCTGCAAGTCTAGTCAAAGCCTCGTA8210.13118955852556358No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8150.13023080413926227No Hit
CTCCATCACTGTGTACGAGGCTTTGACTAGACTTGCAGGTGATGGAGGCC8100.12943184215067782No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC7830.12511744741232192No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7620.12176180706026729No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7480.1195247134922309No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA7470.119364921094514No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGTTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG7400.11824637431049581No Hit
CTCCTTACCTGTCACCCTTGGACAGCCGGCCTCCATCACCTGCAAGTCTA7360.11760720471962825No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA7340.11728761992419448No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7320.11696803512876071No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT7230.11552990354930874No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7220.11537011115159185No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7180.1147309415607243No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6980.11153509360638657No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6910.11041654682236839No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6910.11041654682236839No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG6880.10993716962921772No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6850.10945779243606707No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAACTAC6810.10881862284519953No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6800.10865883044748263No Hit
GTATTACTGTGCGAGAGAGGGAAAAACCACCAGTGACTACTGGGGCCAGG6770.10817945325433197No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT6730.10754028366346442No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGACTTCACACTGAA6700.10706090647031377No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6570.10498360529999425No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG6470.1033856813228254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6330.10114858775478897No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6330.10114858775478897No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6320.1009887953570721No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC6270.10018983336848766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG200.007221797551.9940843
CGTCTTA200.0076297451.27651631
ATCGCAT200.007749030451.07570621
GGTATCA4500.042.3806951
GCCCAAT350.001420766239.0052329
CGCTTGA450.001575641838.19098369
GTATCAA11400.036.4458051
GTGGTAT1450.035.230261
CTAGACC400.002769989434.045034
ATATACC400.002769989434.045034
AATGGTA6200.032.81663570
TATCAAC13000.032.2118382
ACGAGAC851.354183E-732.047522
CTACGCT751.8233332E-631.8338728
AACAACG450.004092107531.4379755
TGGTATC1650.030.9500272
TAGGGTG553.5188356E-430.9500245
ATCAACG13450.030.8809953
TCAACGC13700.030.3174724
GAAGTCG450.004927243630.26708622
Produced by FastQC (version 0.11.5)