FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625812
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16560.26461621061916357No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16380.26173994746025964No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14270.22802375154199664No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13940.2227506024173394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11830.1890344064990764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11390.18200354099953342No Hit
GTGTATTACTGTGCGAGAGAGGGAAAAACCACCAGTGACTACTGGGGCCA11270.18008603222693076No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11020.1760912222840086No Hit
GTTTATTACTGCATGCAAGCTACACATTTTCCTCATCGGACGTTCGGCCA10460.167142848011863No Hit
GTGATGGAGACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTT10450.1669830556141461No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9750.15579758777396405No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9710.1551584181830965No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAGTCTGTCCCTGCCCCACTGCCA9170.14652962870638467No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8510.13598333045707017No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8150.13023080413926227No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8100.12943184215067782No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8080.12911225735524406No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7960.12719474858264143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7930.12671537138949077No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA7870.12575661700318944No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7720.12335973103743615No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT7660.12240097665113484No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7630.1219215994579842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7540.12048346787853222No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAACTAC7370.11776699711734515No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT7270.11616907314017628No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGTTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG7240.11568969594702562No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA7140.11409177196985676No Hit
CTCCATCACTGTGTACGAGGCTTTGACTAGACTTGCAGGTGATGGAGGCC7100.11345260237898921No Hit
CCCTTGGACAGCCGGCCTCCATCACCTGCAAGTCTAGTCAAAGCCTCGTA7070.11297322518583855No Hit
CTCCTTACCTGTCACCCTTGGACAGCCGGCCTCCATCACCTGCAAGTCTA7050.11265364039040478No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7050.11265364039040478No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC7050.11265364039040478No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6970.1113753012086697No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA6950.11105571641323593No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6940.11089592401551902No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT6840.10929800003835018No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6780.10833924565204886No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG6710.10722069886803065No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6610.10562277489086179No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6600.1054629824931449No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6570.10498360529999425No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT6490.10370526611825917No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6390.10210734214109028No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG6300.10066921056163833No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6280.10034962576620456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCG350.005360775656.05633570
TACGTAT400.00908962249.04929770
TGCACGA459.814573E-538.46202542
GGTATCA3850.037.9321821
GTGGTAT1450.035.1336561
TGGTATC1550.032.8722272
GTATCAA10450.032.8250351
GCACGAC553.170615E-431.50487543
CCCTATG652.5826284E-531.350031
GGCATAC450.00465753830.61739339
TATCAAC11450.029.963012
TCAACGC11700.029.3180874
AATGGTA6500.029.17804170
ACGACAG605.2134314E-428.94087245
CAACGCA11950.028.7070335
GATATAT605.630848E-428.56249836
ATCAACG11900.028.5376633
AACGCAG12300.028.1663046
CACGACA658.3803E-426.68410144
CTATGGG659.487701E-426.1229363
Produced by FastQC (version 0.11.5)