FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530883
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20670.38935132599838385No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGCCTGCATAGGAGCTG19400.36542891748275985No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGCCTGCATAGGAGCTGCAGTAATAAT18430.34715747160862187No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG17470.32907439115586673No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15930.30006611626290536No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15820.2979940966276939No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT15800.29761736578492815No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTT15440.2908362106151449No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC14220.2678556292064353No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14110.26578360957122377No Hit
GGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAAGTCATGATTCTTGAGGTCAATAAG13850.2608861086152693No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13350.251467837546126No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13310.25071437586059453No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13220.2490190870681487No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12810.24129610479145122No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12770.24054264310591975No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12160.22905235240156496No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12080.227545429030502No Hit
GATTATTACTGCAGCTCCTATGCAGGCAGCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT12040.22679196734497054No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11960.2252850439739076No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11900.22415485144561043No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11780.22189446638901603No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11770.22170610096763316No Hit
CCCCTGAGGGCCGCTTATTGACCTCAAGAATCATGACTTTGGGGGCTTTGCCTGGGTGCTGCTGGTACCAGGAGA11700.2203875430179531No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCA11470.21605513832614717No Hit
TCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCAGCTCCTAT11420.21511331121923288No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10980.2068252326783868No Hit
CCCAAGTGTTGCTGCCTGCATAGGAGCTGCAGTAATAATCAGCCGCATCC10740.20230446256519796No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10340.19476984570988334No Hit
GTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG10200.1921327298105232No Hit
GTTATAACCACCAACGTCACCGCTGGTTCCAGTGCAGGAGATGGTGACTG10030.18893051764701452No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9960.18761195969733444No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9950.18742359427595157No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCCAGAGAAG9840.18535157464074006No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9820.18497484379797433No Hit
GTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCCAGAGAAGCGATCAGGGAC9720.18309118958414566No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9570.18026570826340266No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9530.1795122465778712No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9190.17310782225085378No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9160.17254272598670517No Hit
CCTATGCAGGCAGCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC9120.17178926430117372No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGGCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG9060.1706590717728765No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8820.16613830165968774No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC8800.165761570816922No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8770.16519647455277342No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8680.1635011857603276No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCAGCTCCTATGCAGGCAGCAACA8660.1631244549175619No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC8450.1591687810685217No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGC8450.1591687810685217No Hit
ACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC8250.15540147264086437No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8220.15483637637671577No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG8180.1540829146911843No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT8000.1506923371062927No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7960.14993887542076126No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGGCTGGCAACACGG7910.14899704831384694No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG7840.1476784903641669No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7810.14711339410001825No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT7760.14617156699310394No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC7710.14522973988618962No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7650.1440995473578924No Hit
GCCTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTATTGAC7580.14278098940821235No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA7480.1408973351943837No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7360.13863695013778932No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7270.13694166134534352No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA7250.13656493050257779No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC7220.13599983423842918No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7180.1352463725528977No Hit
CCTGAGGGCCGCTTATTGACCTCAAGAATCATGACTTTGGGGGCTTTGCC7150.13468127628874912No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC6990.13166742954662328No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT6970.13129069870385754No Hit
GCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAGA6960.13110233328247467No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6900.12997214075417748No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGC6880.12959540991141175No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6840.12884194822588027No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT6770.12752339027620022No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6750.1271466594334345No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC6730.12676992859066877No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6670.12563973606237155No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC6600.1243211781126915No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCC6590.12413281269130863No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6410.12074223510641705No Hit
TCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCCAGAGAAGCGATCAGGGACC6370.11998877342088558No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6350.11961204257811985No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT6210.11697492667875974No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGCCTGCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAG6200.11678656125737685No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT6190.116598195835994No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC6140.11565636872907968No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC6080.11452617620078248No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6040.113772714515251No Hit
CTCCAAGGCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCAG5980.1126425219869538No Hit
GTCACCATCTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTA5970.11245415656557095No Hit
GTCATGATTCTTGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTT5940.11188906030142234No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC5870.11057050235174229No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5820.10962867524482796No Hit
CTGCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGC5800.10925194440206222No Hit
GGCAAAGCCCCCAAAGTCATGATTCTTGAGGTCAATAAGCGGCCCTCAGG5780.1088752135592965No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC5760.10849848271653076No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTG5760.10849848271653076No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG5750.1083101172951479No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA5730.10793338645238217No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG5700.10736829018823356No Hit
CAGCAGCACCCAGGCAAAGCCCCCAAAGTCATGATTCTTGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCG5690.1071799247668507No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGGCTGGCAACACGGCCTCCCTGACCGT5680.10699155934546782No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5620.10586136681717065No Hit
CTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAAGTCATGA5610.10567300139578777No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC5610.10567300139578777No Hit
GCTCCAGGCTGAGGATGCGGCTGATTATTACTGCAGCTCCTATGCAGGCA5580.10510790513163917No Hit
GGCTGAGGATGCGGCTGATTATTACTGCAGCTCCTATGCAGGCAGCAACA5480.10322425091781051No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5390.10152896212536472No Hit
GTGTTGCTGCCTGCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAGC5380.10134059670398186No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG5350.10077550043983326No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT5320.10021040417568466No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT5310.10002203875430178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC150.001742268174.4437356
AGGGGGG2950.071.2101170
GTATAGT150.002948121665.197891
AACTGTA350.003111080564.307170
ATACTTA251.76281E-459.55498556
AGTATGA200.004424062658.84667664
CCCATAT200.004424062658.84667664
GGGCGTT400.005279217456.26870370
ATGTCGT200.00775234551.0687937
ATATCGT200.00775234551.0687937
CGTATGC2800.049.86882441
CTTGAAA2800.049.47348857
TAGTTCG200.00922128648.875397
GCTAATC2400.047.41961333
TCTCGTA2400.047.039938
CTTATAC2850.046.896731
ATGCCGT3100.046.80650344
TCGTATG2900.046.65827640
GCCGTCT2850.046.2957546
TTTAAGT656.1879377E-446.16919370
Produced by FastQC (version 0.11.5)