FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530883
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30750.5792236707523127No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC19100.35977795484127384No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGCCTGCATAGGAGCTG18900.35601064641361657No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGCCTGCATAGGAGCTGCAGTAATAAT17430.3283209294703353No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17250.32493035188544367No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG16600.3126865994955574No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGC14660.2761437077472814No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14610.2752018806403671No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14480.27275313016238983No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTT14470.27256476474100694No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13670.2574955310303777No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13420.25278639549580606No Hit
GGTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAAGTCATGATTCTTGAGGTCAATAAG13350.251467837546126No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13310.25071437586059453No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12190.22961744866571354No Hit
GATTATTACTGCAGCTCCTATGCAGGCAGCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT11980.22566177481667338No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11890.22396648602422756No Hit
TCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCAGCTCCTAT11720.22076427386071884No Hit
CCCCTGAGGGCCGCTTATTGACCTCAAGAATCATGACTTTGGGGGCTTTGCCTGGGTGCTGCTGGTACCAGGAGA11670.21982244675380452No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11660.21963408133242163No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAGCAGCA11640.21925735048965592No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11410.21492494579785No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11370.21417148411231854No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11310.21304129158402135No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11180.21059254110604408No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10860.2045648476217924No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10780.20305792425072944No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10560.1989138849803064No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10490.19759532703062635No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10460.19703023076647774No Hit
CCCAAGTGTTGCTGCCTGCATAGGAGCTGCAGTAATAATCAGCCGCATCC10390.1957116728167977No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10280.19363965318158616No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC10220.19250946065328894No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10030.18893051764701452No Hit
GTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG9780.18422138211244285No Hit
GTTATAACCACCAACGTCACCGCTGGTTCCAGTGCAGGAGATGGTGACTG9680.1823377278986142No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9500.1789471503137226No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9430.17762859236404255No Hit
CCTATGCAGGCAGCAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC9050.17047070635149364No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCCAGAGAAG8900.16764522503075066No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8670.16331282033894473No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8650.162936089496179No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8410.15841531938299022No Hit
GTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCCAGAGAAGCGATCAGGGAC8350.15728512685469304No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC8250.15540147264086437No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8200.15445964553395003No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8190.1542712801125672No Hit
ACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC8110.15276435674150424No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8100.1525759913201214No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8090.1523876258987385No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCAGCTCCTATGCAGGCAGCAACA7900.14880868289246407No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGGCTGGCAACACGG7870.14824358662831547No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGGCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG7790.14673666325725254No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7770.1463599324144868No Hit
GCCTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTATTGAC7680.144664643622041No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC7620.14353445109374383No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGC7550.14221589314406374No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGC7550.14221589314406374No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC7510.1414624314585323No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7500.14127406603714943No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7480.1408973351943837No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG7370.13882531555917216No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7330.1380718538736407No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT7270.13694166134534352No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT7070.1331743529176862No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC7010.13204416038938901No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC6910.13016050617556035No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6900.12997214075417748No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA6730.12676992859066877No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA6640.12507463979822298No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC6630.12488627437684009No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6460.12168406221333136No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT6450.12149569679194851No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC6440.12130733137056564No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC6430.12111896594918278No Hit
CCTGAGGGCCGCTTATTGACCTCAAGAATCATGACTTTGGGGGCTTTGCC6410.12074223510641705No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGTGTTGCTGCCTGCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAG6400.12055386968503419No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT6350.11961204257811985No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC6280.11829348462843979No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6260.11791675378567405No Hit
CTCCAAGGCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGCGGCTGATTATTACTGCAG6210.11697492667875974No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6180.11640983041461112No Hit
GTGTTGCTGCCTGCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAGC6140.11565636872907968No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT6140.11565636872907968No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC6130.1154680033076968No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6110.11509127246493106No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT6100.1149029070435482No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6090.11471454162216534No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCC6060.11414944535801674No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC5990.11283088740833669No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5920.1115123294586566No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC5890.11094723319450801No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC5810.10944030982344508No Hit
CTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGCCCCCAAAGTCATGA5750.1083101172951479No Hit
CTGCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGC5730.10793338645238217No Hit
GGCTGAGGATGCGGCTGATTATTACTGCAGCTCCTATGCAGGCAGCAACA5720.1077450210309993No Hit
GTCATGATTCTTGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTT5720.1077450210309993No Hit
TCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGCCTTGGAGCCAGAGAAGCGATCAGGGACC5690.1071799247668507No Hit
GCATAGGAGCTGCAGTAATAATCAGCCGCATCCTCAGCCTGGAGCCCAGA5690.1071799247668507No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5640.10623809765993639No Hit
GTCACCATCTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTA5630.1060497322385535No Hit
GGCAAAGCCCCCAAAGTCATGATTCTTGAGGTCAATAAGCGGCCCTCAGG5590.10529627055302204No Hit
CAGCAGCACCCAGGCAAAGCCCCCAAAGTCATGATTCTTGAGGTCAATAAGCGGCCCTCAGGGGTCCCTGATCG5570.1049195397102563No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG5490.1034126163391934No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5490.1034126163391934No Hit
GTGCTGCTGGTACCAGGAGACATAGTTATAACCACCAACGTCACCGCTGGTTCCAGTGCAGGAGATGGTGACTGA5450.10265915465366192No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGGCTGGCAACACGGCCTCCCTGACCGT5410.10190569296813046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)