FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520794
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG48820.32101652163277866No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATATA40230.2645328690144753No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTAGCAGTTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGATGGGG35830.23560061388984962No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32750.21534803530261168No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC29970.19706811047387088No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28560.18779663780893402No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28260.18582398405043682No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT27840.1830622687885407No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC26130.17181814236510665No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT25210.16576867083904853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23200.15255189065711727No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22890.15051348177333682No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT22320.14676543963219213No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG21870.14380645899444633No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC21460.14111049885783347No Hit
CTCCAGAGACAACTCCAAGAGCACACTATATCTGCAAATGAACAGCCTGAGAGTCGAGGACACGGCCGTATATTA20840.13703368109027259No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20780.13663915033857313No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19740.12980061730911616No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT19020.12506624828872287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18940.12454020728645694No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG18470.12144971639814467No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA18320.12046338951889606No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA18230.11987159339134688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17850.11737289863058376No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA17330.11395363211585527No Hit
GTAATGGAGACACCTACTTGAACTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTCATTTATAAGATTT16810.11053436560112678No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT16250.10685207858526533No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16230.10672056833469885No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC16180.10639179270828264No Hit
CAGTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATA16070.105668486330167No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15620.10270950569242118No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA15600.10257799544185471No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG15560.10231497494072175No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15560.10231497494072175No Hit
CTCCAGCCCCATCCCTGGAGCCTGGCGGACCCAGGTCATGGCATAACTGC15530.10211770956487204No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15380.10113138268562344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)