FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511321
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70470.4662808232003658No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65920.43617471073319297No Hit
GGCTAAGACTTTCCCGATCACCTTCGGCCAAGGGACGCGACTGGAGAGTAAACGAACTGTGGCTGCACCAT62710.414935013805803No Hit
GTCTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCGTCAGGCTGCAGGCTGTTGATAGTGAGAGTGAAATGTGTC54560.36100868048548257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50570.33460793570657726No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46350.30668534348427634No Hit
CACTATCAACAGCCTGCAGCCTGACGATTTTGCAACTTACTTTTGTCAACAGGCTAAGACTTTCCCGATCACCTT42260.2796229259038947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40060.26506612427141557No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38940.2576553888948807No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38230.25295751200439875No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38180.25262667560366064No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37020.24495127110653528No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36910.2442234310249113No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33840.22391007601958815No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33170.21947686824969678No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32180.21292630751508118No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32090.21233080199375248No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31570.20889010342607559No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31120.20591257581943212No Hit
GTGCAGCCACAGTTCGTTTACTCTCCAGTCGCGTCCCTTGGCCGAAGGTG31030.20531707029810345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30680.20300121549293632No Hit
CTCCTAATCAATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCGCGGTT30510.20187637173042658No Hit
CATTTCACTCTCACTATCAACAGCCTGCAGCCTGACGATTTTGCAACTTACTTTTGTCAACAGGCTAAGACTTTC29730.19671532387891122No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGTGACAT29230.19340695987152962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29070.19234828338916748No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28760.19029709770459088No Hit
GTTGATAGTGAGAGTGAAATGTGTCCCATATCCACTGCCGGTGAACCGCG28680.18976775946340982No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27090.17924716191993628No Hit
CTATCAACAGCCTGCAGCCTGACGATTTTGCAACTTACTTTTGTCAACAG26970.17845315455816468No Hit
GTTGGAGACACAGTCACCATCAGCTGTCGGGCGAATCAGGGAATTAGCAGCTGGGTGGCCTGGTACCAGCAGAAA26170.17315977214635409No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25810.17077775006103932No Hit
GGATATGGGACACATTTCACTCTCACTATCAACAGCCTGCAGCCTGACGA25220.166873880532329No Hit
CTCGTGTACAGCCTCTGGATTCACCTTCAGCAACTCTGGCATGTGCTGGGTCCGCCAGTCTCCAGGCAAGGGG24700.16343318196465212No Hit
GTACTATCATAACTGTCCTTCGCACAGAAATAAACGGCCGTGTCCTCGGC24660.1631685128440616No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTACTCTCCAGTCGCGTCCCTTGGCCGAAGGTGATCGGGAAAGT24570.1625730073227329No Hit
GTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT24150.15979398155653232No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23560.15589011202782202No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23530.15569161018737912No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23500.15549310834693622No Hit
CGACTGGAGAGTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT23330.15436826458442648No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22580.14940571857335402No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22470.1486778784917301No Hit
CCTTCGGCCAAGGGACGCGACTGGAGAGTAAACGAACTGTGGCTGCACCA22140.14649435824685822No Hit
CTCCAGTCGCGTCCCTTGGCCGAAGGTGATCGGGAAAGTCTTAGCCTGTT21980.14543568176449612No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21850.14457550712257689No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21810.14431083800198635No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21620.14305365967918132No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21580.1427889905585908No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20900.1382896155085518No Hit
CCCCTAAACTCCTAATCAATGCTGCATCCAGTTTACAAAGTGGGGTCCCA20690.13690010262545152No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20680.1368339353453039No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGTGACATCC20530.1358414261430894No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20410.1350474187813178No Hit
GATTAGGAGTTTAGGGGCCTTCCCTGGTTTCTGCTGGTACCAGGCCACCC20020.13246689485556015No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19950.1320037238945267No Hit
GTCTCCATCTTCTGTGTTTGCATCTGTTGGAGACACAGTCACCATCAGCTGTCGGGCGAATCAGGGAATTAGCAG19370.12816602164596402No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19280.12757051612463532No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19160.12677650876286375No Hit
GTTCGTTTACTCTCCAGTCGCGTCCCTTGGCCGAAGGTGATCGGGAAAGT19130.12657800692242083No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19080.1262471705216827No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18970.12551933044005872No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18900.1250561594790253No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18870.12485765763858239No Hit
GCCTGGTACCAGCAGAAACCAGGGAAGGCCCCTAAACTCCTAATCAATGCTGCATCCAGTTTACAAAGTGGGGTC18790.12432831939740135No Hit
CTCCAGCCCCTTGCCTGGAGACTGGCGGACCCAGCACATGCCAGAGTTGCTGAAGGTGAATCCAGAGGCTGTACA18590.12300497379444869No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18230.12062295170913392No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18060.11949810794662419No Hit
GTATTATTGGTAGCGGTGTTGACACTTACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT17820.117910093223081No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17700.11711608586130941No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17580.11632207849953781No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17540.11605740937894729No Hit
GTTTAGGGGCCTTCCCTGGTTTCTGCTGGTACCAGGCCACCCAGCTGCTA17400.11513106745688045No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17320.11460172921569937No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17310.11453556193555174No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17300.11446939465540411No Hit
GTCACCATCAGCTGTCGGGCGAATCAGGGAATTAGCAGCTGGGTGGCCTGGTACCAGCAGAAACCAGGGAAGGCC17220.11394005641422306No Hit
GTGTTGACACTTACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCC17100.11314604905245146No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17060.11288137993186094No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16940.11208737257008934No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16910.11188887072964644No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16870.11162420160905591No Hit
GAGTGAAATGTGTCCCATATCCACTGCCGGTGAACCGCGATGGGACCCCA16810.11122719792817012No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16660.11023468872595563No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC16630.11003618688551274No Hit
CCCATATCCACTGCCGGTGAACCGCGATGGGACCCCACTTTGTAAACTGG16580.10970535048477459No Hit
TGATAGTACTGGTCCCCAGATTTGGTTTGACTCCTGGGGCCAGGGAGACC16520.10930834680388879No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16340.1081173357612314No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16310.1079188339207885No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16210.10725716111931217No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16080.10639698647739297No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15790.10447813535311161No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15730.10408113167222582No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15720.10401496439207819No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15670.10368412799134004No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15530.10275778606927317No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15500.10255928422883027No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15230.1007727676648442No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15120.10004492758322024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)