FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511321
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68930.45609106205763034No Hit
GGCTAAGACTTTCCCGATCACCTTCGGCCAAGGGACGCGACTGGAGAGTAAACGAACTGTGGCTGCACCAT58640.3880049307857166No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56710.37523464571722354No Hit
GTCTTAGCCTGTTGACAAAAGTAAGTTGCAAAATCGTCAGGCTGCAGGCTGTTGATAGTGAGAGTGAAATGTGTC56430.3733819618730898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49780.3293807205749143No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46000.3043694886791092No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40320.266786473555254No Hit
CACTATCAACAGCCTGCAGCCTGACGATTTTGCAACTTACTTTTGTCAACAGGCTAAGACTTTCCCGATCACCTT38850.257059883373552No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37790.25004615167790295No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35810.23694503020867175No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35770.23668036108808124No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34440.2278801128284461No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34170.22609359626446002No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33140.2192783664092539No Hit
GTGCAGCCACAGTTCGTTTACTCTCCAGTCGCGTCCCTTGGCCGAAGGTG31310.20716975414223718No Hit
CTCCTAATCAATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCGCGGTT30840.20405989197529845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30480.20167786988998368No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30040.19876650956348785No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29790.19711232755979702No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29770.19697999299950175No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29190.19314229075093908No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28620.18937075578252402No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28030.18546688625381372No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27930.18480521345233739No Hit
GTTGATAGTGAGAGTGAAATGTGTCCCATATCCACTGCCGGTGAACCGCG27530.1821585222464321No Hit
CATTTCACTCTCACTATCAACAGCCTGCAGCCTGACGATTTTGCAACTTACTTTTGTCAACAGGCTAAGACTTTC27460.18169535128539868No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27060.17904866007949338No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26190.17329210670664935No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGTGACAT25850.17104241918162985No Hit
GTTGGAGACACAGTCACCATCAGCTGTCGGGCGAATCAGGGAATTAGCAGCTGGGTGGCCTGGTACCAGCAGAAA25530.16892506621690562No Hit
CTCGTGTACAGCCTCTGGATTCACCTTCAGCAACTCTGGCATGTGCTGGGTCCGCCAGTCTCCAGGCAAGGGG25440.16832956069557692No Hit
CTATCAACAGCCTGCAGCCTGACGATTTTGCAACTTACTTTTGTCAACAG23880.15800746499254625No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23520.1556254429072315No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTACTCTCCAGTCGCGTCCCTTGGCCGAAGGTGATCGGGAAAGT23410.15489760282560752No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23320.15430209730427885No Hit
GGATATGGGACACATTTCACTCTCACTATCAACAGCCTGCAGCCTGACGA23250.15383892634324542No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22940.15178774065866882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22800.15086139873660195No Hit
GTACTATCATAACTGTCCTTCGCACAGAAATAAACGGCCGTGTCCTCGGC22560.14927338401305876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22540.14914104945276352No Hit
GTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT22460.14861171121158245No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21820.14437700528213399No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21690.14351683064021475No Hit
CGACTGGAGAGTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT21010.13901745559017575No Hit
CTCCAGTCGCGTCCCTTGGCCGAAGGTGATCGGGAAAGTCTTAGCCTGTT20850.13795877910781362No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20840.137892611827666No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20660.1367016007850086No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20620.1364369316644181No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20510.13570909158279412No Hit
GATTAGGAGTTTAGGGGCCTTCCCTGGTTTCTGCTGGTACCAGGCCACCC20250.1339887422989557No Hit
GTTCGTTTACTCTCCAGTCGCGTCCCTTGGCCGAAGGTGATCGGGAAAGT19940.13193755661437906No Hit
CCTTCGGCCAAGGGACGCGACTGGAGAGTAAACGAACTGTGGCTGCACCA19880.1315405529334933No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19630.12988637092980249No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19350.12803368708566876No Hit
GTCTCCATCTTCTGTGTTTGCATCTGTTGGAGACACAGTCACCATCAGCTGTCGGGCGAATCAGGGAATTAGCAG19080.1262471705216827No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18870.12485765763858239No Hit
CCCCTAAACTCCTAATCAATGCTGCATCCAGTTTACAAAGTGGGGTCCCA18740.12399748299666319No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18550.12274030467385817No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18520.12254180283341527No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGTGACATCC18510.12247563555326764No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18410.12181396275179131No Hit
GTGTTGACACTTACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCC18380.12161546091134842No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18360.12148312635105316No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18340.12135079179075789No Hit
GTATTATTGGTAGCGGTGTTGACACTTACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT17910.11850559874440969No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17840.11804242778337627No Hit
CTCCAGCCCCTTGCCTGGAGACTGGCGGACCCAGCACATGCCAGAGTTGCTGAAGGTGAATCCAGAGGCTGTACA17790.11771159138263809No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17600.11645441305983308No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17460.11552807113776624No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17300.11446939465540411No Hit
GAGTGAAATGTGTCCCATATCCACTGCCGGTGAACCGCGATGGGACCCCA17240.11407239097451832No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17140.11341071817304199No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17130.11334455089289436No Hit
GTTTAGGGGCCTTCCCTGGTTTCTGCTGGTACCAGGCCACCCAGCTGCTA17120.11327838361274672No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17060.11288137993186094No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16980.11235204169067989No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16910.11188887072964644No Hit
CCCATATCCACTGCCGGTGAACCGCGATGGGACCCCACTTTGTAAACTGG16730.11069785968698907No Hit
GCCTGGTACCAGCAGAAACCAGGGAAGGCCCCTAAACTCCTAATCAATGCTGCATCCAGTTTACAAAGTGGGGTC16530.10937451408403642No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16390.10844817216196956No Hit
GGGCTGGAGTGGGTCGCAAGTATTATTGGTAGCGGTGTTGACACTTACTA16390.10844817216196956No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16310.1079188339207885No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16270.10765416480019796No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16080.10639698647739297No Hit
GTGGGGTCCCATCGCGGTTCACCGGCAGTGGATATGGGACACATTTCACT15930.10540447727517847No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15850.1048751390339974No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15610.10328712431045423No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15400.10189761142735394No Hit
GCCTGAGAGCCGAGGACACGGCCGTTTATTTCTGTGCGAAGGACAGTTAT15300.10123593862587763No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC15270.10103743678543473No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15250.10090510222513947No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15220.10070660038469657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3100.052.5575261
TGGTATC3350.047.6110422
AATCGAG607.342926E-444.60622470
GGTATCA10800.042.7435461
GTATCAA31200.039.6006361
ATCAACG36250.034.0646743
CGGGATA400.00280802833.95352617
CGATTCG400.002810309433.947916
TCAACGC36750.033.6000984
CAACGCA37200.033.196945
AACGCAG37550.032.884256
CGCAGAG39850.030.9862888
AGAGTAC39900.030.86039411
CAGAGTA40550.030.36370510
AATGTCG450.005023794730.14993910
GCAGAGT41200.030.0493189
GCAGGCT18450.029.17033844
TATATGC1650.028.78806713
TGCAGGC18800.028.40888843
TGTTAGA18950.028.24668370
Produced by FastQC (version 0.11.5)