FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682323
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT40950.6001556447606192No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31240.4578476762471732No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29060.42589799845527704No Hit
ATACAATACTTATTCGACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT27200.3986381816236592No Hit
GTATTGTATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC25080.36756785276181514No Hit
GAGTATTGGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTGATCTATAAGGGGT22300.32682468566939704No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21930.32140203393407524No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21220.3109964049284576No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19080.2796329597565962No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18220.2670289584258482No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18180.2664427258058134No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18170.26629616765080466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17910.2624856556205785No Hit
AGTTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACCATCAGCAGC17200.25208002661496093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16900.2476832819647No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16280.23859667635416074No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16250.23815700188913463No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA16120.23625174587402153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15650.2293635125886127No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15540.22775137288351702No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAACTGGTTGGCCTGGTATCAGCAGAAA14630.2144145807777255No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14430.21148341767755155No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA13980.20488830070216013No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13890.20356927730708183No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13660.20019843974188178No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13270.19448267169654254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13100.19199118306139468No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12880.18876690365120333No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATA12450.1824649029858293No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11880.1741110881503335No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11780.17264550660024652No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC11610.17015401796509866No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11240.16473136622977683No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11210.16429169176475072No Hit
CTCTAAACTAGACCCCTTATAGATCAGGAGTTTAGGGGCTTTTCCTGGTT11020.16150708681958545No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10770.157843132944368No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10590.1552050861542114No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9820.14392010821854168No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9630.14113550327337637No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9530.1396699217232894No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9510.13937680541327202No Hit
CCAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA9440.13835089832821112No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9270.13585940969306326No Hit
GAGTGAATTCTGTCCCAGATCGACTGCCGCTGAACCTTGATGGGACCCCA9250.13556629338304585No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8840.12955740902768922No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8800.12897117640765446No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8720.12779871116758484No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8630.12647968777250657No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8570.12560033884245436No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8550.12530722253243698No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8530.12501410622241957No Hit
ACTTATTACTGCCAACAATACAATACTTATTCGACTTTTGGCCAGGGGAC8380.12281573389728911No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8340.12222950127725432No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAA8300.12164326865721953No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8210.12032424526214124No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8190.12003112895212385No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8180.11988457079711516No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8170.11973801264210644No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8150.11944489633208906No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8130.11915178002207165No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTC8120.11900522186706296No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATACAATACTTATTCGACTTTTGG8090.11856554740203686No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8030.1176861984719847No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7960.1166602913869238No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7860.11519470983683681No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7670.11241010489167155No Hit
GTATTGGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCT7610.11153075596161935No Hit
GTCTAGTTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACCATCAG7570.11094452334158456No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7560.11079796518657586No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7550.11065140703156716No Hit
CATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG7440.10903926732647147No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7380.1081599183964193No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7330.1074271276213758No Hit
GGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7320.1072805694663671No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7300.1069874531563497No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC7280.10669433684633231No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7210.10566842976127142No Hit
GGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTGATCTATAAGGGGTCTAGTTTAGAGACTGGGGTCCCAT7120.10434940636619315No Hit
CTCCTGATCTATAAGGGGTCTAGTTTAGAGACTGGGGTCCCATCAAGGTT7110.10420284821118443No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7090.10390973190116705No Hit
GTATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGA7040.10317694112612355No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6950.10185791773104527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT309.332918E-657.2782639
TACGTAT651.4569103E-553.11900370
CTTGAAA7950.052.4774157
CCGTCTT7500.051.90701747
GCCGTCT7550.051.8166446
ATGCCGT7500.051.77791644
TCGTATG7600.051.73956340
GCTTGAA8100.051.23732456
TGCTTGA8100.051.03380655
CGTATGC7700.050.77879341
CTGCTTG8200.050.66452454
ATCTCGT6950.050.47489537
CGTCTTC7900.049.92648748
GTATGCC7950.049.8081742
GATCTCG7100.049.26722736
TATACAC7850.048.878443
TGTCGTA352.388898E-548.86863338
ATCTCCG7850.048.86769510
CGAGACT7800.048.8357123
TCCGAGC7800.048.75697713
Produced by FastQC (version 0.11.5)