FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682323
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68090.9979144774542262No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31210.4574080017821472No Hit
ATACAATACTTATTCGACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT25610.37533543497727617No Hit
GTATTGTATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC25300.3707921321720065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24870.3644901315066325No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22310.32697124382440573No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22140.32447975518925787No Hit
GAGTATTGGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTGATCTATAAGGGGT20200.2960474731175704No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18170.26629616765080466No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17790.26072695776047417No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17650.2586751435903524No Hit
AGTTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACCATCAGCAGC16270.23845011819915202No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16040.23507928063395195No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15630.22907039627859532No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15270.22379430269828218No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA15180.22247527930320388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15130.22174248852816042No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14990.21969067435803863No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14270.20913848719741235No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13950.20444862623713403No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAACTGGTTGGCCTGGTATCAGCAGAAA13660.20019843974188178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13590.1991725326568209No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13390.19624136955664692No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13280.19462922985155126No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12960.18993936889127291No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12850.18832722918617723No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12810.18774099656614243No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12700.18612885686104674No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12530.18363736822589888No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12000.17586978601043787No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATA11890.1742576463053422No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10690.1566706677042984No Hit
CTCTAAACTAGACCCCTTATAGATCAGGAGTTTAGGGGCTTTTCCTGGTT10550.15461885353417665No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10500.15388606275913314No Hit
GAGTGAATTCTGTCCCAGATCGACTGCCGCTGAACCTTGATGGGACCCCA10430.15286015567407224No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10210.1496358762638809No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC9900.14509257345861123No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9780.14333387559850688No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9650.14142861958339378No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9630.14113550327337637No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9320.13659220046810674No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9260.13571285153805454No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9230.13527317707302844No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9050.1326351302828719No Hit
CCAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8930.1308764324227675No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8890.1302901998027327No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8870.12999708349271533No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8680.12721247854755005No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8290.12149671050221084No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8260.12105703603718473No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG8170.11973801264210644No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTAA8150.11944489633208906No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8080.11841898924702816No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8020.11753964031697597No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7960.1166602913869238No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7950.11651373323191508No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7840.1149015935268194No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7830.11475503537181071No Hit
ACTTATTACTGCCAACAATACAATACTTATTCGACTTTTGGCCAGGGGAC7800.11431536090678461No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7790.11416880275177592No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTC7760.11372912828674982No Hit
CTCCTGATCTATAAGGGGTCTAGTTTAGAGACTGGGGTCCCATCAAGGTT7750.11358257013174113No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7670.11241010489167155No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7620.11167731411662804No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7550.11065140703156716No Hit
GTCCCAGATCGACTGCCGCTGAACCTTGATGGGACCCCAGTCTCTAAACT7460.10933238363648888No Hit
GTATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGA7410.10859959286144538No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7380.1081599183964193No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATACAATACTTATTCGACTTTTGG7340.10757368577638451No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7260.1064012205363149No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7230.10596154607128883No Hit
GTATTGGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCT7210.10566842976127142No Hit
GTCTAGTTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACCATCAG7200.10552187160626272No Hit
GCTTGGTCCCCTGGCCAAAAGTCGAATAAGTATTGTATTGTTGGCAGTAA6830.10009921987094089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCA150.002639110767.0468131
CTACGGC307.674149E-444.207725
GTGGTAT2650.038.6636351
GTATCAA19300.035.619921
AATGGTA3000.033.32184270
CTAGTTC400.00321343433.0293668
TATCAAC21400.031.803952
TCAACGC21300.031.7841644
ATCAACG21350.031.7097263
CAACGCA21700.031.200575
AACGCAG22000.030.7751066
TATCAAT554.2044008E-430.02449412
ACGGGTA1550.029.84391416
CGCAGAG22750.029.7627248
GACAGGT902.9025432E-729.378821
AGAGTAC23400.029.3529811
CGGGTAC803.863357E-628.91129317
GCAGAGT24000.028.483659
GACGTGA500.006838280728.30362147
GCGGCAT550.00736838827.87368264
Produced by FastQC (version 0.11.5)