FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208915
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT30031.4374267046406435No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC6390.30586602206639063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.29725007778283036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.2623076370772802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.2426824306536151No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.20247469066366702No Hit
GTATCAACGCAGAGTACGGGAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC3830.18332814781131082No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.1737548763851327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.17231888567120598No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG3350.16035229638848336No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.14886437067706962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.1459923892492162No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG3050.1459923892492162No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG3030.1450350621065984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.14168441710743604No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.1392910992508915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2830.13546179068042027No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.13498312710911137No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCC2770.13258980925256683No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG2710.12971782782471342No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGG2680.1282818371107867No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.12253787425507982No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.11918722925591749No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.11918722925591749No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.11775123854199077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.11679391139937297No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC2410.11535792068544624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.11200727568628388No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.11104994854366608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.11104994854366608No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC2260.10817796711581264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.10578464925926812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10530598568795921No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.10004068640356124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3800.0113.3081470
AAGGGGG3750.075.8851769
AAAGGGG4350.060.93856468
CTAAGGC150.004592226458.2839415
ACCTAAG150.00459658258.26998513
TTACACT200.004708730657.9096144
AAAAGGG4600.056.467467
AAAAAGG4700.054.96962466
TCTTCTG5350.052.3400450
TTCTGCT5100.051.9583352
GCCGTCT5250.051.13984746
CTTCTGC5400.050.79420551
TCTGCTT5300.050.41573753
TATGCCG5150.050.3368643
CTTGAAA5600.050.27662757
CCGTCTT5400.050.17498847
GTATGCC5100.049.31068442
CGAGAGA250.0092371348.8369149
ATACGGG302.4747142E-548.5583231
CTGCTTG5600.048.21892554
Produced by FastQC (version 0.11.5)