FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284695
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGAAGAAGCA51280.39916089032805446No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49050.3818026846839133No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41890.3260696118533971No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39690.3089449246708363No Hit
GTCTATTACTGTGCGAGAGTGGCCACCTCTTACTATGAGAATAGTGGTTA34920.2718154892795566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32550.253367530814707No Hit
CTCCCAGACTCCTCATCTATGATACATCCAGCAGGGCCACCGGCATCCCA31050.24169160773568824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29250.22768050004086574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28900.2249561179890947No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGAAGCAAC28140.21904031696239187No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26920.2095438995247899No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26410.20557408567792357No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25740.20035884003596185No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25640.19958044516402723No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATACAGCCACCCTCTCCT25330.19716742106103005No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCTCCAGGAACTTAGCCTGGTACCAACAAAAAGCTGGGCAGGCTCCCAGACT25240.19646686567628893No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23680.18432390567410942No Hit
GTCTGGGAGCCTGCCCAGCTTTTTGTTGGTACCAGGCTAAGTTCCTGGAG23640.1840125477253356No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23420.1823000790070795No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23330.18159952362233836No Hit
GGCCTGGAGTGGGTCTCAGTTCTTTACAGCGATGGCAGCACATATTCCGC22980.17887514157056733No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21670.16867816874822428No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21520.1675105764403224No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGGCCAGTTGCTTCG21230.16525323131171213No Hit
GTTTATTACTGTCAGCAGCGAAGCAACTGGCCTCCGAGGTTCACTTTTGGCCAGGGGACCAACCTGGAGATCAAA21220.16517539182451865No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGG20710.16120557797765228No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20710.16120557797765228No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGAAGCAACTGG20600.16034934361852424No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20290.15793631951552703No Hit
GTCTTTGTCTCCAGGGGATACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCTCCAGGAACTTAGCCTGGTA20110.1565352087460448No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT19940.155211937463756No Hit
CTCCAGGCCCGTCCCTGGAGCCTGGCGGACCCAGCTCATGTATTTGCTAC19350.15061940771934193No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA19180.14929613643705314No Hit
GAGTGAAGTCTGTCCCAGACGCACTGCCACGGAACCTGGCTGGGATGCCG19030.14812854412915127No Hit
CCCTTAGACTCTCCTGTGCAGTCTCTGGATTCACCGTCAGTAGCAAATAC19010.14797286515476435No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18930.1473501492572167No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17870.1390991636147101No Hit
CTCCTGTGCAGTCTCTGGATTCACCGTCAGTAGCAAATACATGAGCTGGGTCCGCCAGGCTCCAGGGACGGGC17800.1385542872043559No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17440.1357520656653914No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17310.13474015233187644No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17010.13240496771607269No Hit
GTCCCAGACGCACTGCCACGGAACCTGGCTGGGATGCCGGTGGCCCTGCT16990.13224928874168576No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16700.12999194361307548No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATACAGC16520.1285908328435932No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGATACAGCCACCCTCTCCTGC16250.12648916668936985No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15740.12251935284250347No Hit
GTCAGAGTGTCTCCAGGAACTTAGCCTGGTACCAACAAAAAGCTGGGCAGGCTCCCAGACTCCTCATCTATGATA15710.1222858343809231No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA15680.12205231591934272No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT15680.12205231591934272No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15580.12127392104740813No Hit
GGCCAAAAGTGAACCTCGGAGGCCAGTTGCTTCGCTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCTAGG15300.1190944154059913No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15150.11792682309808944No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14870.11574731745667259No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14660.11411268822560997No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14460.1125558984817408No Hit
ATGATACATCCAGCAGGGCCACCGGCATCCCAGCCAGGTTCCGTGGCAGTGCGTCTGGGACAGACTTCACTCTCA14290.11123262719945201No Hit
CAGTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCACTGCCA13960.10866392412206788No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGATCTTGTGTTGACACAGTC13900.10819688719890715No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13900.10819688719890715No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13850.10780768976293983No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13580.10570602360871646No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13240.10305948104413888No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13190.10267028360817158No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12890.10033509899236785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG14200.050.79332770
TCGTATG3350.049.3255340
CTAGCGC3750.046.85656728
ACTAGCG3900.045.89753327
TATACAC4350.044.9281163
CGTATGC3550.044.7531441
CGCTCAT3600.044.32339532
GGTATCA9600.044.2433321
GCGCTCA3900.043.4691731
ATCTCGT3500.042.9713737
GAGACTA4100.042.7631324
CTTATAC4350.042.6208341
TAGCGCT4150.042.38660429
GACTAGC4150.042.2989926
GCCGTCT4050.042.23467646
AATACGG405.748905E-542.1201066
ACGAGAC4300.041.54855722
CACGAGA4300.041.5372321
CCCACGA4400.040.58688419
CGAGACT4350.040.3023423
Produced by FastQC (version 0.11.5)