FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284695
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49100.3821918821198806No Hit
GTAATAGACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGAAGAAGCA44550.346774915446857No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38000.2957900513351418No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37150.2891736949236978No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36320.2827130174866408No Hit
GTCTATTACTGTGCGAGAGTGGCCACCTCTTACTATGAGAATAGTGGTTA35060.272905242100265No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32640.2540680861994481No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31490.24511654517220038No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28840.22448908106593393No Hit
CTCCCAGACTCCTCATCTATGATACATCCAGCAGGGCCACCGGCATCCCA27550.2144477872179778No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26470.20604112260108431No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGAAGCAAC25940.20191562977983102No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24940.19413168106048517No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24700.19226353336784216No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCTCCAGGAACTTAGCCTGGTACCAACAAAAAGCTGGGCAGGCTCCCAGACT24360.18961699080326458No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATACAGCCACCCTCTCCT24160.18806020105939542No Hit
GTCTGGGAGCCTGCCCAGCTTTTTGTTGGTACCAGGCTAAGTTCCTGGAG23710.1845574241356898No Hit
GGCCTGGAGTGGGTCTCAGTTCTTTACAGCGATGGCAGCACATATTCCGC22780.17731835182669817No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22740.17700699387792435No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21700.16891168720980465No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21530.16758841592751586No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20790.16182829387519995No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGCGAAGCAACTGG20720.16128341746484573No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGGCCAGTTGCTTCG20590.1602715041313308No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20560.16003798566975042No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20400.15879255387465507No Hit
GTTTATTACTGTCAGCAGCGAAGCAACTGGCCTCCGAGGTTCACTTTTGGCCAGGGGACCAACCTGGAGATCAAA20360.15848119592588122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20270.15778064054114013No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20110.1565352087460448No Hit
GAGTGAAGTCTGTCCCAGACGCACTGCCACGGAACCTGGCTGGGATGCCG19270.1499966918217943No Hit
GTCTTTGTCTCCAGGGGATACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCTCCAGGAACTTAGCCTGGTA19030.14812854412915127No Hit
GTAATAAACTGCAAAATCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA18850.146727433359669No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT18840.14664959387247556No Hit
GTCCCAGACGCACTGCCACGGAACCTGGCTGGGATGCCGGTGGCCCTGCT18760.14602687797492792No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18610.14485928566702602No Hit
CTCCTGTGCAGTCTCTGGATTCACCGTCAGTAGCAAATACATGAGCTGGGTCCGCCAGGCTCCAGGGACGGGC18530.14423656976947835No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGG18400.1432246564359634No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18360.14291329848718956No Hit
CTCCAGGCCCGTCCCTGGAGCCTGGCGGACCCAGCTCATGTATTTGCTAC17910.13941052156348394No Hit
CCCTTAGACTCTCCTGTGCAGTCTCTGGATTCACCGTCAGTAGCAAATAC17580.13684181848609983No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATACAGC17180.13372823899836148No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17110.13318336258800728No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16580.12905786976675399No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGATACAGCCACCCTCTCCTGC16010.12462101899672684No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15610.12150743950898851No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15320.11925009438037823No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15180.11816034155966981No Hit
GTCAGAGTGTCTCCAGGAACTTAGCCTGGTACCAACAAAAAGCTGGGCAGGCTCCCAGACTCCTCATCTATGATA14910.11605867540544644No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA14630.11387916976402959No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14490.11278941694332117No Hit
GGCCAAAAGTGAACCTCGGAGGCCAGTTGCTTCGCTGCTGACAGTAATAAACTGCAAAATCCTCAGGCTCTAGG14460.1125558984817408No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGATCTTGTGTTGACACAGTC14460.1125558984817408No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14200.11053207181471088No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13920.10835256617329404No Hit
ATGATACATCCAGCAGGGCCACCGGCATCCCAGCCAGGTTCCGTGGCAGTGCGTCTGGGACAGACTTCACTCTCA13860.1078855292501333No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13770.10718497386539218No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13610.10593954207029685No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13470.10484978924958843No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13410.10438275232642767No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13040.10150269130026972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA409.050038E-568.14992570
TCTGGAG14500.049.3499470
GGTATCA10750.040.0986251
ATTCCGT350.00152127738.469536
GTATCAA26650.037.404381
CCGTTTA500.002193876335.70461769
TATCAAC29200.034.0146182
ACGTATC400.002924005333.67395417
ATTGTCT12500.033.43105364
AATTGTC12700.033.0796963
ATCAACG30100.032.8741843
TCAACGC30450.032.500124
CAACGCA30950.032.0850875
TACGTAT850.00371201432.0705570
GAATTGT13100.031.90769862
AACGCAG31200.031.7188646
GGAATTG13150.031.38105261
TCGTTTT450.005185749429.95458424
ACGCAGA33600.029.5522637
GTGTTCC14150.029.43073753
Produced by FastQC (version 0.11.5)