FastQCFastQC Report
Wed 8 May 2024
EGAF00004692101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810193
Total Bases59.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37570.4637166699786347No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31760.3920053616854256No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC27400.33819102362029785No Hit
TCTCTGATGCGTCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGC27040.3337476379084983No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25650.3165912319657168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23710.29264632007435265No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22320.27548991413157115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22280.27499620460803786No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCACCCCAGAGCCTGAAGATT22270.27487277722715453No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22200.2740087855609713No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21910.27042939151535494No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTT19750.24376907724455776No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19510.2408068201033581No Hit
GTTTATTACTGTCAACATAGGGCCACTTTCGGCCCTGGGACCAAAGTGGA19250.23759770820039175No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCTAGGGGAAAGAGGCACCCTCTCCT19120.2359931522489086No Hit
GTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGATGCGT18430.22747666296795951No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTGGGGTGCTGATAGTGAGAGTGA18360.22661267130177623No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18360.22661267130177623No Hit
CATCAGAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGTTGGTACCAG18180.2243909784458765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17770.21933045582966035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17250.21291223202372767No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGCTCCTTAGCCTGGTACCAAC16890.20846884631192816No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAAATCCACTTTGGTCC16740.20661743559867835No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC16720.20637058083691173No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16570.20451917012366191No Hit
GTGCAGCCACAGTTCGTCTGAAATCCACTTTGGTCCCAGGGCCGAAAGTG16350.20180376774422884No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16350.20180376774422884No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15780.1947684070338796No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCTAGGGGAAAGAGG15710.19390441536769634No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGAGTCCAACCTCAGGGA14610.1803274034705311No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14450.178352565376398No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14380.17748857371021473No Hit
ACATAGGGCCACTTTCGGCCCTGGGACCAAAGTGGATTTCAGACGAACTG14330.17687143680579812No Hit
CACTATCAGCACCCCAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAAC14140.17452631656901504No Hit
GTCTTTGTCTCTAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGA14020.1730451879984152No Hit
GTATTATTGTGTGAGGGACTTCGGTGACTACAGAATTGACTACTGGGGCC14000.17279833323664853No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA13730.1694657939527989No Hit
CCCCAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACATAGGGCCACT13530.1669972463351325No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT12910.15934474872036664No Hit
GACTTCACTCTCACTATCAGCACCCCAGAGCCTGAAGATTTTGCAGTTTA12710.15687620110270023No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12670.15638249157916695No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12650.1561356368174003No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12620.15576535467475033No Hit
ACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGATGCGTCCAACA12560.1550247903894504No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12540.15477793562768377No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12480.15403737134238385No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12120.14959398563058432No Hit
CCTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATC11920.14712543801291791No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT11900.14687858325115127No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11890.14675515587026794No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGAGTCCAACCTCAGGGAGA11870.14650830110850133No Hit
ATGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTGGGGTGCTGATAG11640.14366947134818495No Hit
GCCACAGTTCGTCTGAAATCCACTTTGGTCCCAGGGCCGAAAGTGGCCCT11540.14243519753935174No Hit
CTGTTGGTACCAGGCTAAGGAGCTGCCAACATTCTGACTGGCCCTGCAGG11370.14033693206433528No Hit
GTGTATTATTGTGTGAGGGACTTCGGTGACTACAGAATTGACTACTGGGG11310.13959636777903536No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11310.13959636777903536No Hit
CTTTCGGCCCTGGGACCAAAGTGGATTTCAGACGAACTGTGGCTGCACCA11310.13959636777903536No Hit
CTCCAGGCACCCTGTCTTTGTCTCTAGGGGAAAGAGGCACCCTCTCCTGC11280.1392260856363854No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11250.13885580349373544No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11200.13823866658931885No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC10930.1349061273054692No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10880.13428899040105258No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG10690.1319438701642695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.13182044278338617No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10530.12996903207013638No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGATGTTGTGTTGACACAGTC10400.1283644761186532No Hit
CCTCATCTCTGATGCGTCCAACAGGGCCACTGGCATCCCAGGCAGGTTCA10310.12725362969070333No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10060.12416794516862031No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10010.1235508082642037No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9980.12318052612155375No Hit
GTCAGAATGTTGGCAGCTCCTTAGCCTGGTACCAACAGAGACCTGGCCAG9980.12318052612155375No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9790.12083540588477067No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9750.12034169636123739No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9750.12034169636123739No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9660.1192308499332875No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCA9640.11898399517152086No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9590.11836685826710426No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9560.1179965761244543No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9520.117502866600921No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9390.11589831064943786No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9310.11491089160237128No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9270.114417182078838No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9250.11417032731707137No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9220.1138000451744214No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC9210.11367661779353808No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC9170.1131829082700048No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCA9130.11268919874647151No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9030.11145492493763831No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9020.11133149755675499No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC9020.11133149755675499No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC9010.11120807017587167No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT8840.10910980470085523No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8830.1089863773199719No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8590.10602412017877222No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG8590.10602412017877222No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG8550.10553041065523892No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8510.10503670113170566No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT8500.10491327375082232No Hit
CCCAGATACCACCGGAGATGTTGTGTTGACACAGTCTCCAGGCACCCTGT8480.1046664189890557No Hit
GCCCAGACCATGGACCAGTTCTCCCTGAGGTTGGACTCTGTGACCGCCGC8440.10417270946552241No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA8390.10355557256110581No Hit
CTACAGAATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG8320.10269158089492257No Hit
CTCACTATCAGCACCCCAGAGCCTGAAGATTTTGCAGTTTATTACTGTCA8290.10232129875227261No Hit
TTGTAGGAGGTGGTCCTATAATAATCGATACTTCCAAGCCACTCCGGCCC8270.10207444399050597No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT8260.10195101660962264No Hit
GTCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTG8230.10158073446697269No Hit
GCCCTATGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTGGGGTGCT8200.10121045232432271No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACTATCAGCACCC8150.10059331541990611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)