FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004692102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810193
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34250.4227387795253723No Hit
TCTCTGATGCGTCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT23370.28844978912431973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22960.28338926650810364No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22890.28252527484192036No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCACCCCAGAGCCTGAAGATT21830.2694419724682884No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21580.2663562879462054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21550.26598600580355547No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20880.25771637128437297No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20450.2524089939063902No Hit
GTTTATTACTGTCAACATAGGGCCACTTTCGGCCCTGGGACCAAAGTGGA18930.23364803201212553No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18390.2269829534444262No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTT17690.21834303678259379No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17620.21747904511641053No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTGGGGTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16530.2040254606001286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16160.19945864750744577No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15770.19464497965299626No Hit
GTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGATGCGTCCAACAGGGCCACTGGCATCCCAGG15620.19279356893974647No Hit
CATCAGAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGTTGGTACCAGGCTAAGGAGCTGCCAACATTCTGA15480.19106558560738No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCTAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAG15410.19020159394119673No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15390.1899547391794301No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15390.1899547391794301No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15280.18859703798971358No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCTAGGGGAAAGAGG14920.18415365227791403No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14790.18254909632643085No Hit
GTGCAGCCACAGTTCGTCTGAAATCCACTTTGGTCCCAGGGCCGAAAGTGGCCCTATGTTGACAGTAATAAACTG14520.17921655704258121No Hit
GTCTTTGTCTCTAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAGCTCCTTAGCCTGGTA14370.1773651463293314No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAAATCCACTTTGGTCC14100.17403260704548174No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGCTCCTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCT14030.1731686153792985No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13540.16712067371601583No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13250.16354127967039953No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12840.15848075705418338No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12790.15786362014976676No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12590.15539507253210036No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12260.1513219689629508No Hit
ACATAGGGCCACTTTCGGCCCTGGGACCAAAGTGGATTTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTT12180.15033454991588424No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12110.14947055824970099No Hit
CACTATCAGCACCCCAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACATAGGGCCACTTTCGGCCCTGGGAC12000.14811285705998448No Hit
GTATTATTGTGTGAGGGACTTCGGTGACTACAGAATTGACTACTGGGGCC11990.14798942967910114No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11910.14700201063203458No Hit
CCCCAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACATAGGGCCACT11510.14206491539670177No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11230.1386089487319688No Hit
GACTTCACTCTCACTATCAGCACCCCAGAGCCTGAAGATTTTGCAGTTTA11150.13762152968490224No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11020.13601697373341906No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10980.13552326420988578No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10890.1344124177819359No Hit
CTGTTGGTACCAGGCTAAGGAGCTGCCAACATTCTGACTGGCCCTGCAGGAGAGGGTGCCTCTTTCCCCTAGAGA10890.1344124177819359No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10770.13293128921133607No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10600.13083302373631964No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10510.12972217730836974No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10500.1295987499274864No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10390.1282410487377699No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10370.12799419397600326No Hit
ACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGATGCGTCCAACAGGGCCACTGGCATCCCAGGCAGGTT10310.12725362969070333No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGAGTCCAACCTCAGGGA10070.12429137254950365No Hit
GTGTATTATTGTGTGAGGGACTTCGGTGACTACAGAATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC9740.12021826898035406No Hit
CTCCAGGCACCCTGTCTTTGTCTCTAGGGGAAAGAGGCACCCTCTCCTGCAGGGCCAGTCAGAATGTTGGCAGCT9670.11935427731417081No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9580.11824343088622094No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9520.117502866600921No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9500.11725601183915438No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9450.11663887493473778No Hit
GCCACAGTTCGTCTGAAATCCACTTTGGTCCCAGGGCCGAAAGTGGCCCT9390.11589831064943786No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9250.11417032731707137No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9110.11244234398470487No Hit
CCTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATC9100.11231891660382157No Hit
CTTTCGGCCCTGGGACCAAAGTGGATTTCAGACGAACTGTGGCTGCACCA9080.11207206184205491No Hit
GTCAGAATGTTGGCAGCTCCTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGATG8890.10972694160527183No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8850.10923323208173856No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGATGTTGTGTTGACACAGTC8800.10861609517732196No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8800.10861609517732196No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCA8760.10812238565378868No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8660.10688811184495547No Hit
CCTCATCTCTGATGCGTCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT8610.10627097494053886No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8460.10441956422728907No Hit
GGCTAAGGAGCTGCCAACATTCTGACTGGCCCTGCAGGAGAGGGTGCCTC8440.10417270946552241No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA8350.10306186303757252No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8330.10281500827580589No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8270.10207444399050597No Hit
CTACTGGGGCTGGGTCCGTCAGGCCCCTGGGAAGGGGCCGGAGTGGCTTGGAAGTATCGATTATTATAGGACCAC8200.10121045232432271No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8160.10071674280078943No Hit
GGCCGAAAGTGGCCCTATGTTGACAGTAATAAACTGCAAAATCTTCAGGC8140.1004698880390228No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8120.10022303327725617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)