FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690398
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT19720.2856323454007688No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT17900.2592707394864991No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG16940.24536571658666453No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC15980.2314606936868299No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA13520.1958290725060038No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT13320.1929321927352049No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG12200.17670966601873123No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC12030.1742473182135522No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA10230.14817540027636233No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT10110.14643727241388302No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA9680.14020898090666545No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT9430.13658788119316684No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT9380.13586366125046712No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA9200.13325646945674813No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC9110.13195287355988863No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC8890.1287663058120099No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT8710.12615911401829089No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.11544065886633506No Hit
GTATCAACGCAGAGTACGGGGACCACGGGTGACGGGGAATCAGGGTTCGA7760.11239893510699625No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT7700.11152987117575659No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG7600.11008143129035716No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7250.10501189169145912No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC7230.10472220371437924No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG7130.1032737638289798No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT6960.10081141602380075No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC6930.10037688405818093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG451.534754E-461.2414770
AGGGGGG4100.050.41218670
GCAATCG200.00942872548.602524
TCGTATG3350.047.44855540
TCTCGTA3250.046.14658438
CTCGTAT3300.045.75817539
CCGTCTT3500.045.2503647
CGTATGC3500.044.76717441
GCCGTCT3600.043.806346
CGTCTTC3750.042.46637348
TATACAC3600.042.2378353
ATCTCGT3550.041.9459837
ATGCCGT3850.041.4338644
TTATACA3650.040.7758182
ACTCGCT3700.040.7621929
CGAGACA3700.040.2568723
TGCCGTC3900.040.2075145
TCGCTAA3800.039.9741331
GGTATCA22150.039.897611
CACTCGC3700.039.74476628
Produced by FastQC (version 0.11.5)