FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191818
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT23381.2188637145627628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.2945500422275282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.2658770292673263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.24241729139079754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.2325120687318187No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.22729879364814565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.22625613863141103No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.22208551856447256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.21374427843059568No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT3870.20175374573814764No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.18819923052059764No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.18037931789508804No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC3390.1767300253365169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.17308073277794578No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.17203807776121116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.16473949264406887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.16421816513570156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3110.16213285510223233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.1527489599516208No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.14805701237631505No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2590.1350238246671324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.1277252395499901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.12563992951652087No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.12355461948305164No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.11625603436590935No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT2200.11469205184080744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2180.11364939682407282No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.1126067418073382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.10895744924876707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.10530815669019591No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.1042655016734613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.10165886413162477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1930.10061620911489015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.10009488160652284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3600.0114.2394470
CACACAT254.0619329E-4107.28573670
TCGTCTG150.001082192883.8977250
AAGGTAC255.980944E-574.0439660
AAGGGGG3650.073.7614669
AAATGGG451.2284181E-768.10216567
CAGCGTA400.002626693667.0535870
AATAGCG250.003291307563.3751168
GTGTGTG250.003757618161.29194667
AAAGGGG4000.060.73447868
TTAAAGG200.00401996260.265246
TGCTGTA450.004188761659.60318870
AAAACGG351.9396955E-458.3732867
GAGCAAA250.004655404558.06605563
ATACGGG202.0819758E-457.566131
CAAGTAT150.0048374857.5211142
GTATGTT150.0048374857.5211145
TGAGCAA250.004902809457.3119562
CTTGCGA200.005268076456.28851741
TCTGCTT4400.054.95665753
Produced by FastQC (version 0.11.5)