FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273486
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6800.24864161236772633No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5370.1963537438845133No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4610.16856438720812034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4590.16783308834821528No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4010.14662542141096802No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC3990.14589412255106293No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT3910.14296892711144263No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCACTTTTGGCCAGGGGA3760.13748418566215456No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3690.13492463965248677No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3660.13382769136262915No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT3460.1265147027635784No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3360.122858208464053No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3360.122858208464053No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3030.11079177727561923No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2940.10750093240604638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.10713528297609384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.10567268525628368No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2860.10457573696642605No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC2780.10165054152680576No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG2750.10055359323694814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCT201.0471587E-466.157188
CGAGGGT250.00640840653.57604269
TCCGCGA200.00680875652.7654743
CCGAAAC200.00748771551.51249338
TACGTAT651.841575E-551.030270
CCGACTT200.00863333849.69062823
GTATCAA17000.038.3462831
CCCTACA350.001652180837.8179441
TTATAGT350.001653667537.8110244
TCGCTCT450.00210679635.98845368
GTACGTA751.0543552E-535.7173669
TATCAAC19400.033.24912
ATCAACG19500.032.921013
TCAACGC19550.032.4923364
CAACGCA19650.032.158615
AACGCAG19800.032.0762066
GGTATCA6500.031.5634371
ATGGCAA2557.2759576E-1231.21847770
AATGGTA850.004223465531.21847770
CGCAGAG20200.031.1135278
Produced by FastQC (version 0.11.5)