FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473667
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC89580.6078713847836723No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCT82360.5588779554675514No Hit
GACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGAC79200.5374348478998309No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG63860.4333407750869091No Hit
ATATAACCTTGTGTCCTGGTACCAACAACACCCCGGCGAGGCCCCCAGGC63760.4326621957335001No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG63400.43021931006122827No Hit
GTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTC63030.4277085664536153No Hit
CTCCTGTACTGGAACCAGCAGCAATGTTGGCAAATATAACCTTGTGTCCTGGTACCAACAACACCCCGGCGAGGC60770.41237267306657477No Hit
CTCCTGGACAGTCGATCACCATCTCCTGTACTGGAACCAGCAGCAATGTT60610.41128694610112054No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56060.3804115855210166No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC54200.3677900095476115No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA52110.35360770106136596No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT50140.34023968779921104No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC47560.32273234048126204No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC46040.31241793430944714No Hit
GTCTGGAGTCTTGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC45830.3109929176672885No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT44840.3042749820685406No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG44200.2999320742067238No Hit
GTCGATCACCATCTCCTGTACTGGAACCAGCAGCAATGTTGGCAAATATAACCTTGTGTCCTGGTACCAACAACA43660.2962677456983158No Hit
ACTCCAGACTGAGGACGAGGGTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCTGGGAC42920.29124625848309016No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG42000.2850033284317285No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAC41540.28188186340604765No Hit
GTCTGGTCCCAGAGCCGAACACTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCAGT41230.2797782674104801No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA40760.2765889444494584No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39950.2710924516868465No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG39270.2664781120836661No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA38660.262338778027872No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38180.25908159713150936No Hit
AGGTTATATTTGCCAACATTGCTGCTGGTTCCAGTACAGGAGATGGTGAT37770.25629942178253295No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG37360.25351724643355655No Hit
ACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACC35890.24354212993844604No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35520.24103138633083324No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT33810.2294276793875414No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33800.22935982145220052No Hit
CTTCAAAGAGTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT32980.22379547075424772No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32750.2222347382414073No Hit
GTACTGGAACCAGCAGCAATGTTGGCAAATATAACCTTGTGTCCTGGTAC32210.2185704097329994No Hit
CTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCA32100.21782397244424961No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGC31230.21192033206959238No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACA30300.2056095440828898No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA30250.20527025440618538No Hit
GGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG29150.19780588151868772No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28640.19434512681630248No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC28110.1907486562432354No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27990.18993436101914477No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC27870.1891200657950541No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27710.1880343388295999No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT27110.18396286270914664No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC26460.17955209691198892No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT26090.1770413533043761No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26080.1769734953690352No Hit
GAGTAAAGACCCTCGTCCTCAGTCTGGAGTCTTGAGATTGTCAGGGAGGC25440.17263058750721838No Hit
GTAAAGACCCTCGTCCTCAGTCTGGAGTCTTGAGATTGTCAGGGAGGCCG25260.1714091446710824No Hit
TCTCAAGACTCCAGACTGAGGACGAGGGTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCT24610.16699837887392469No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACT24530.1664555153911976No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23530.15966972185710884No Hit
GTAAACGGCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGC23490.15939829011574527No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23070.156548256831428No Hit
CAACAACACCCCGGCGAGGCCCCCAGGCTCATAATTTATGAGGTCAGTAAACGGCCCTCAGGCGTTTCTGATCG22680.1539017973531334No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22660.1537660814824516No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC22620.15349464974108804No Hit
GGTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGT22240.1509160481981343No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22220.15078033232745255No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCTTTGAAGAC21870.14840530459052148No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21840.1482017307844988No Hit
ATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCC21560.14630170859495395No Hit
GTCTTGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG21530.1460981347889313No Hit
GCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCAGTCTGGAGTCTTGA20970.1422980904098416No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC20860.1415516531210918No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20790.1410766475737056No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG20430.13863376190143364No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGTA20390.13836233016007007No Hit
CCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTACCTGAGCCT20340.13802304048336564No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAG20070.13619087622916168No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC19960.13544443894041192No Hit
TAATTTATGAGGTCAGTAAACGGCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCT19540.13259440565609465No Hit
GTACAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG19470.1321194001087084No Hit
AGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG19110.12967651443643646No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19110.12967651443643646No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19080.1294729406304138No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC18770.12736934463484628No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG18770.12736934463484628No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC18510.1256050383159832No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGAAAC18500.1255371803806423No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18470.12533360657461964No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18220.12363715819109745No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18030.12234785741962058No Hit
GTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCT17900.12146570426018904No Hit
GGTACCAACAACACCCCGGCGAGGCCCCCAGGCTCATAATTTATGAGGTC17850.12112641458348461No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTACCTGAGCCTGACGC17820.12092284077746193No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17610.1194978241353033No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17590.11936210826462151No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17520.11888710271723531No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCT17510.11881924478189443No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17380.11793709162246288No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT17120.1161727853035998No Hit
CTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTCC17030.11556206388553181No Hit
GTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC16900.11467991072610026No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT16880.11454419485541849No Hit
GCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG16610.11271203060121453No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACT16580.11250845679519185No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16500.11196559331246476No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16460.11169416157110121No Hit
CTCCCTGACAATCTCAAGACTCCAGACTGAGGACGAGGGTCTTTACTCCT16350.11094772428235143No Hit
GTGTTGTTGGTACCAGGACACAAGGTTATATTTGCCAACATTGCTGCTGGTTCCAGTACAGGAGATGGTGATCGA16160.10965842351087457No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC16150.10959056557553369No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCC15900.1078941171920115No Hit
GTCCTGGTACCAACAACACCCCGGCGAGGCCCCCAGGCTCATAATTTATG15890.1078262592566706No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15810.10728339577394351No Hit
CAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGG15780.10707982196792085No Hit
CCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT15760.10694410609723908No Hit
GTCCCAGAGCCGAACACTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCAGTCTGGAGT15670.10633338467917108No Hit
GGCTCATAATTTATGAGGTCAGTAAACGGCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACA15530.10538337358439866No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT15520.10531551564905775No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC15510.10524765771371687No Hit
GCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15470.10497622597235332No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15470.10497622597235332No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG15390.10443336248962623No Hit
GGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC15270.10361906726553556No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCGGGGCAGACTGGGCC15260.10355120933019468No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGT15180.10300834584746757No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG15070.10226190855871782No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG15060.10219405062337691No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC15020.10192261888201339No Hit
CTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCAGT14990.10171904507599071No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT14930.1013118974639454No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA14910.10117618159326361No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG14880.10097260778724096No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT14790.10036188636917297No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC14780.10029402843383206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)