FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473667
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC82320.5586065237261878No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCT76950.5221668124481311No Hit
GACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGAC76480.5189774894871093No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG68490.46475899914974006No Hit
ATATAACCTTGTGTCCTGGTACCAACAACACCCCGGCGAGGCCCCCAGGC62800.42614783394077493No Hit
CTCCTGTACTGGAACCAGCAGCAATGTTGGCAAATATAACCTTGTGTCCTGGTACCAACAACACCCCGGCGAGGC61920.4201763356307769No Hit
GTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTC60940.41352625796736986No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG59670.4049083001790771No Hit
CTCCTGGACAGTCGATCACCATCTCCTGTACTGGAACCAGCAGCAATGTT59230.40192255102407803No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53360.3620899429789769No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC51920.3523184002898891No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA48380.32829669117921484No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT47110.3196787333909221No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG47020.3190680119728541No Hit
GTCTGGAGTCTTGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC46920.3183894326194452No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG46920.3183894326194452No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC45890.3114000652793338No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT44750.3036642606504726No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC43750.2968784671163838No Hit
GTCGATCACCATCTCCTGTACTGGAACCAGCAGCAATGTTGGCAAATATAACCTTGTGTCCTGGTACCAACAACA42930.291314116418431No Hit
GTCTGGTCCCAGAGCCGAACACTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCAGT41840.28391760146627426No Hit
ACTCCAGACTGAGGACGAGGGTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCTGGGAC41800.2836461697249107No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG41050.2785568245743441No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38480.26111733519173597No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37960.25758872255400983No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG36910.2504636393432166No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACA36840.2499886337958304No Hit
AGGTTATATTTGCCAACATTGCTGCTGGTTCCAGTACAGGAGATGGTGAT36760.24944577031310328No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36280.24618858941674068No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35390.2401492331714017No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA34730.23567060943890308No Hit
GTACTGGAACCAGCAGCAATGTTGGCAAATATAACCTTGTGTCCTGGTAC33710.22874910003413254No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAC33260.22569549294379257No Hit
CTTCAAAGAGTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT32220.21863826766834027No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32010.2172132510261816No Hit
CTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCA31670.21490608122459146No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31300.2123953376169786No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGC31050.2106988892334564No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31000.21035959955675196No Hit
ACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCTTTGAAGACCTGCATATGAATAGCAGGAGTAAAGACC30210.20499882266482183No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29660.201266636221073No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC28890.19604157519982468No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28760.1951594220403931No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28750.1950915641050522No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27660.18769504915289545No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC26480.1796878127826707No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT25930.1759556263389219No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA25850.17541276285619478No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC24940.169237690740174No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC24490.16618408364983406No Hit
GTAAAGACCCTCGTCCTCAGTCTGGAGTCTTGAGATTGTCAGGGAGGCCG24440.16584479397312962No Hit
GGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG24330.16509835668437983No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACT24320.16503049874903897No Hit
GAGTAAAGACCCTCGTCCTCAGTCTGGAGTCTTGAGATTGTCAGGGAGGC24280.1647590670076754No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT24120.1636733400422212No Hit
TCTCAAGACTCCAGACTGAGGACGAGGGTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCT23730.16102688056392658No Hit
GGTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGT22760.15444466083586048No Hit
ATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCC22720.1541732290944969No Hit
GTAAACGGCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGC21710.14731957762506728No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21670.1470481458837037No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC21550.14623385065961306No Hit
GTCTTGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG21320.14467311814677264No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21250.14419811259938645No Hit
GCATATGAATAGCAGGAGTAAAGACCCTCGTCCTCAGTCTGGAGTCTTGA21240.14413025466404553No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21230.14406239672870466No Hit
CAACAACACCCCGGCGAGGCCCCCAGGCTCATAATTTATGAGGTCAGTAAACGGCCCTCAGGCGTTTCTGATCG21120.1433159594399549No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACTCTTTGAAGAC21080.14304452769859133No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20850.1414837951857509No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20280.13761589287132034No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCAGAGCCGAACACT20250.13741231906529766No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGTA19960.13544443894041192No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCT19940.13530872306973013No Hit
GGTACCAACAACACCCCGGCGAGGCCCCCAGGCTCATAATTTATGAGGTC19740.1339515643629124No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19650.13334084294484438No Hit
TAATTTATGAGGTCAGTAAACGGCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCT19630.1332051270741626No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC19340.13123724694927688No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC18990.1288622192123458No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18860.12798006605291426No Hit
GTCTTTACTCCTGCTATTCATATGCAGGTCTTCAAAGAGTGTTCGGCTCT18780.12743720257018715No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAG18520.12567289625132408No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG18450.12519789070393786No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTACCTGAGCCTGACGC18390.12479074309189254No Hit
GCTCTGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCG18280.12404430580314277No Hit
CCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTACCTGAGCCT18260.123908589932461No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18180.1233657264497339No Hit
GTACAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG18170.123297868514393No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC18000.12214428361359791No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17930.12166927806621171No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17880.12132998838950727No Hit
TACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17820.12092284077746193No Hit
GTGTTCGGCTCTGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC17820.12092284077746193No Hit
GTGTTGTTGGTACCAGGACACAAGGTTATATTTGCCAACATTGCTGCTGGTTCCAGTACAGGAGATGGTGATCGA17680.1199728296826895No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17660.11983711381200773No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17460.11847995510518997No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC17360.1178013757517811No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17270.1171906543337131No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17090.11596921149757712No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17070.11583349562689536No Hit
AGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG17060.11576563769155447No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGAAAC17000.11535849007950913No Hit
GCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG16780.11386561550200962No Hit
GGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC16460.11169416157110121No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16360.11101558221769234No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16350.11094772428235143No Hit
GTCCTGGTACCAACAACACCCCGGCGAGGCCCCCAGGCTCATAATTTATG16340.11087986634701055No Hit
GGCTCATAATTTATGAGGTCAGTAAACGGCCCTCAGGCGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACA16230.11013342905826079No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16200.10992985525223813No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG16000.10857269654542037No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT15980.10843698067473859No Hit
GCCCCGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15920.10802983306269327No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC15830.10741911164462528No Hit
CTCCCTGACAATCTCAAGACTCCAGACTGAGGACGAGGGTCTTTACTCCT15750.10687624816189817No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT15740.10680839022655729No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15470.10497622597235332No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG15320.10395835694224001No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTCCGTGTCTGGGT15120.10260119823542226No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCC14800.10042974430451385No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCCGACTCAGCCTGCCTC14770.10022617049849118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT200.007662954351.2236941
GTAGTTT901.03223094E-438.13519770
AATGGTA4600.037.30617570
TATCAAC33350.035.7253462
GACAGGT1450.035.3266831
GTATCAA34550.035.2857861
AACGATT501.860308E-434.50427239
ATCAACG35200.033.7392923
TCAACGC35200.033.6400574
CAACGCA35550.033.308865
AACGCAG35900.032.8868336
GGTATCA15250.031.7978741
TTACGAC553.0280178E-431.75664349
ACGCAGA37450.031.4345827
CGCAGAG37550.031.3519338
AGAGTAC37850.031.09815811
GTTCGTT553.4414863E-431.07104727
CAGAGTA38050.031.02752510
CGATTAA450.00456239930.7496241
ACGATTA450.004593976330.70629740
Produced by FastQC (version 0.11.5)