FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947295
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24510.25873671876237075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23630.24944710992879726No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17330.18294195577935068No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16610.17534136673369963No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15910.1679519051615389No Hit
GTTTATTACTGCATGCAAGCTACACAATTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15600.16467942932243917No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA14980.15813447764423963No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14820.1564454578563172No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14760.15581207543584627No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14580.15391192817443353No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14410.15211734464976592No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14210.15000606991486284No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13900.1467335940757631No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT13860.1463113391287825No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGGCAAAGCCTCGTA13830.14599464791854702No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13470.1421943533957215No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA13260.1399775149240733No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13160.13892187755662175No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13100.13828849513615082No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGGTTAGCTCCATGTAGGCTGTACTTATGGAGGTGTTCCTGGT13100.13828849513615082No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12140.1281543764086161No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTT11540.1218205522039069No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11540.1218205522039069No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11510.12150386099367146No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11320.11949814999551353No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11220.118442512628062No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11200.1182313851545717No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11080.11696462031362986No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11040.11654236536664925No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAA11000.11612011041966863No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10980.11590898294617832No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10880.1148533455787268No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10530.11115861479264644No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10410.10989184995170459No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10090.10651381037585968No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10080.10640824663911454No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA10060.10619711916562422No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC9690.10229126090605355No Hit
CCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCT9610.10144675101209231No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGG9480.10007442243440534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)