FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947295
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31550.3330535894309587No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24180.2552531154497807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18680.19719306023994637No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17180.1813584997281734No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16420.17333565573554172No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16320.17228001836809018No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14110.1489504325474113No Hit
GTTTATTACTGCATGCAAGCTACACAATTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14100.14884486881066616No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA14020.14800035891670493No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.14314442702642788No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGGTTAGCTCCATGTAGGCTGTACTTATGGAGGTGTTCCTGGT13510.14261660834270212No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13470.1421943533957215No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA13340.14082202481803452No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12860.13575496545426716No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12680.1338548181928544No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12400.1308990335639901No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT12190.12868219509234188No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12120.12794324893512582No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12040.1270987390411646No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11970.12635979288394852No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11840.12498746430626151No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGGCAAAGCCTCGTA11720.12372069946531966No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11540.1218205522039069No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11440.12076491483645538No Hit
GTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATTT10640.11231981589684313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10470.1105252323721755No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAA10460.11041966863543035No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10440.11020854116194005No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10380.10957515874146914No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA9950.10503591806142754No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9870.1041914081674663No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9830.10376915322048569No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9620.10155231474883747No Hit
GTCTGGGACCCCAGAGAACCGGTTAGAAATCTTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG9580.10113005980185687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGA300.002819721665.9337370
TAACGTC200.007786594351.01529316
TACGATT200.007801242650.9910477
GATTCGC200.007802871550.98835810
GGTATCA9500.046.547651
GTATCAA24050.037.7636681
CGATACA400.001951494436.56617766
CCCGTAG400.002348556235.2120253
GTGGTAT2550.034.6825641
GTATAGA502.0223996E-434.0155871
TATCAAC27300.033.3767172
ATCAACG27400.033.0015073
ACCGATA552.4686934E-432.8796464
CCGATAC450.003594571732.28320765
TCAACGC27900.032.1663974
CAACGCA28200.031.8258825
AACGCAG28800.031.1611966
ACGCAGA29900.030.2421827
ACGGGTA902.2608583E-730.23128716
CGCAGAG29850.030.1805528
Produced by FastQC (version 0.11.5)