FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649973
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATACTGCTGACAGTAATAAACTCCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC55520.8541893278643883No Hit
GTATTACAGCATCCCGTACACCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT51380.7904943743817051No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36530.5620233455851242No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32140.49448207848633713No Hit
GTTTTATTCAGCTCCAACAGTAAGAACTACTTAGCTTGGTACCAGCAGAA25510.3924778413872576No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25470.3918624312086809No Hit
CCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA24790.38140045817287793No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23240.35755331375303284No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22210.34170650165468414No Hit
GTCCCTGACCGCTTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCAC21410.32939829808315113No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21290.32755206754742117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20080.30893590964547757No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19860.305551153663306No Hit
CCCGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTTGGAGCTGAATAAAACACT19160.29478147553821465No Hit
AACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAA19150.2946276229935705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19000.2923198348239081No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATT18550.28539647031492077No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17680.27201129893087866No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17610.27093433111836956No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGGAGTTTATTACTGTCAGC17530.26970351076121624No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17280.2658571971451122No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17280.2658571971451122No Hit
GTCAGGGACCCCGGATTCCCGAATAGAAGCCCAGTAAATGAGCACCCGAGGAGGCTGTCCTGGTTTCTGCTGGTA17030.26201088352900814No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAA16790.25831842245754827No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16470.25339514102893507No Hit
GAATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16130.24816415451103355No Hit
GGCCAAAGGTGTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCC16040.2467794816092361No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15940.24524095616279445No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14890.22908643897515743No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14390.2213938117429493No Hit
CTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATCCGGGGTCCCT14380.22123995919830514No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14190.21831676085006607No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14100.2169320879482686No Hit
CTTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCACCATCACCAGCC14080.2166243828589803No Hit
GTAATAAACTCCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCA13730.21123954379643461No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAGGTG13380.20585470473388892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13170.20262380129636154No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG13110.20170068602849656No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAGGTGTACGGGATG13010.20016216058205494No Hit
CATTTACTGGGCTTCTATTCGGGAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGAGTCTGGGACAGATTTCA12940.19908519276954584No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTTGGAGCTG12800.19693125714452756No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12610.19400805879628846No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAACAGTAAGAACTA12540.19293109098377934No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGGAGTTTATTACTGTCAGCAGTATTACAGCATC12490.19216182826055853No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12490.19216182826055853No Hit
GTCCCAGACTCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTCCCGAAT12460.19170027062662603No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGC12120.18646928410872451No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11730.18046903486760218No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11700.1800074772336697No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATC11350.17462263817112403No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11330.1743149330818357No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11190.17216099745681746No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCACTGAAGCGGTCAGGGACC10950.16846853638535755No Hit
CAGTAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTC10870.16723771602820425No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10780.1658530431264068No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10680.16431451767996516No Hit
GTTTATTACTGTCAGCAGTATTACAGCATCCCGTACACCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA10430.16046820406386111No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10300.158468120983487No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10270.1580065633495545No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10180.15662189044775707No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9950.15308328192094134No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9860.15169860901914387No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9840.15139090392985555No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9840.15139090392985555No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9730.14969852593876976No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9690.14908311576019312No Hit
GCTCCAACAGTAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGG9690.14908311576019312No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9610.14785229540303982No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9570.14723688522446318No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9520.14646762250124237No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9360.14400598178693574No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9280.14277516142978247No Hit
CCACTGAAGCGGTCAGGGACCCCGGATTCCCGAATAGAAGCCCAGTAAAT9250.14231360379584998No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9230.14200589870656166No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9160.14092893089405253No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9090.1398519630815434No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATT9000.1384672901797459No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8690.1336978612957769No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8620.13262089348326775No Hit
GAGTGAAATCTGTCCCAGACTCGCTGCCACTGAAGCGGTCAGGGACCCCG8580.1320054833046911No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCACTGAAGCGGTC8520.13108236803682616No Hit
GTAAATGAGCACCCGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA8500.1307746629475378No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC8460.13015925276896118No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8430.12969769513502868No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8350.12846687477787538No Hit
CAGTAAATGAGCACCCGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGC8320.1280053171439429No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8320.1280053171439429No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8260.12708220187607794No Hit
CAGTAATAAACTCCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCA8190.12600523406356878No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8100.12462056116177134No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8100.12462056116177134No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA8020.12338974080461805No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8020.12338974080461805No Hit
GCTAAGTAGTTCTTACTGTTGGAGCTGAATAAAACACTCTGGCTGGACTT7990.12292818317068556No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7910.12169736281353224No Hit
GCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGAGT7820.1203126899117348No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7810.12015883736709063No Hit
CCCGAATAGAAGCCCAGTAAATGAGCACCCGAGGAGGCTGTCCTGGTTTC7780.11969727973315815No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGGAGTTTATTACTGTCAGCAGTAT7670.11800490174207236No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7620.11723563901885155No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7600.11692793392956323No Hit
CCCGTACACCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGG7590.11677408138491907No Hit
GTAATACTGCTGACAGTAATAAACTCCCACATCTTCAGCCTGCAGGCTGG7590.11677408138491907No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7580.1166202288402749No Hit
CCGTACACCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG7540.11600481866169826No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7540.11600481866169826No Hit
GCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATCCGGGGTCCCTGACCGCTT7510.11554326102776577No Hit
GGTGTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCCACATCTT7500.1153894084831216No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAACAGTAA7430.11431244067061247No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7310.11246621013488252No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7230.11123538977772923No Hit
CCTTGGTCCCCTGGCCAAAGGTGTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCCACATCTTCAGC7150.11000456942057593No Hit
GTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCCACATCTTCAG7070.10877374906342263No Hit
AAGTAGTTCTTACTGTTGGAGCTGAATAAAACACTCTGGCTGGACTTGCA7040.10831219142949015No Hit
CCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAACAGTAAG6930.10661981343840436No Hit
GTGTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCCACATCTTC6930.10661981343840436No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATCCGGGGTCCCTGA6910.10631210834911603No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6900.10615825580447188No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG6880.10585055071518354No Hit
GTGTTTTATTCAGCTCCAACAGTAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTG6850.10538899308125108No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATCCGGGGTC6840.1052351405366069No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC6810.10477358290267441No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6690.10292735236694446No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6510.10015800656334956No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6500.10000415401870538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCCG350.004654207758.0940670
ATGACGG200.007283265251.8828941
GTATGCC900.050.10867742
TCGTATG900.049.78716740
CGTATGC950.047.3317641
ACCGAGG1050.044.79008527
GACCGAG1000.043.62318426
AGACCGA1100.039.63604725
GGTATCA7700.039.143161
ATCTCGT1050.039.048737
GATCTCG1050.038.978336
ATCTCCG1150.037.83982510
GTATCAA18800.037.764361
ATGCCGT1200.037.75307544
CTTATAC1250.037.5078431
GAGACCG1200.036.2994724
ACGAGAC1200.036.28270322
CCGAGGC1300.036.2157528
CTAAACC400.002157450435.8245153
CACGAGA1250.034.82871221
Produced by FastQC (version 0.11.5)