FastQCFastQC Report
Wed 8 May 2024
EGAF00004692116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649973
Total Bases47.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATACTGCTGACAGTAATAAACTCCCACATCTTCAGCCTGCAGGCT61910.9525011038920079No Hit
GTATTACAGCATCCCGTACACCTTTGGCCAGGGGACCAAGGTGGAGATCA53550.8238803765694882No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT41270.6349494517464572No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34080.5243294721473046No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29880.45971140339675653No Hit
GTTTTATTCAGCTCCAACAGTAAGAACTACTTAGCTTGGTACCAGCAGAA27540.4237099079500225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26390.40601686531594394No Hit
CCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA25150.3869391497800678No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25080.38586218196755867No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24240.37293856821744903No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23690.3644766782620201No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22900.3523223272351313No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21770.33493698969034097No Hit
GTCCCTGACCGCTTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCAC21300.32770592009206534No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA19560.3009355773239812No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19390.29832008406503036No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT19130.2943199179042822No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA18930.29124286701139895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18720.2880119635738715No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18670.2872427008506507No Hit
GTCAGGGACCCCGGATTCCCGAATAGAAGCCCAGTAAATGAGCACCCGAG18010.277088432904136No Hit
GTCCCAGACTCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTCCCGAAT18000.2769345803594918No Hit
CCCGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTA17960.2763191701809152No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17690.27216515147552284No Hit
AACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAA17680.27201129893087866No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17640.27139588875230203No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC17180.26431867169867057No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGGAGTTTATTACTGTCAGC17150.26385711406473805No Hit
GGCCAAAGGTGTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCC16980.26124162080578733No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16870.25954924281470154No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16710.25708760210039494No Hit
GTAATAAACTCCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGA16580.2550875190200208No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16050.24693333415388022No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC15760.2424716103591995No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA15680.24124079000204623No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAGGTG15210.2340097204037706No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14590.22447086263583257No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14560.22400930500190008No Hit
GAGTGAAATCTGTCCCAGACTCGCTGCCACTGAAGCGGTCAGGGACCCCG14430.22200922192152595No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG14390.2213938117429493No Hit
CTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATCCGGGGTCCCT14120.21723979303755694No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13550.20847019799283972No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT13350.20539314709995646No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTTGGAGCTG13350.20539314709995646No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACTC13300.20462388437673565No Hit
CTTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCACCATCACCAGCC13200.20308535893029403No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGGAGTTTA13040.20062371821598746No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12890.19831593004632503No Hit
GAATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA12750.19616199442130675No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12720.19570043678737423No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT12600.1938542062516443No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12170.18723854683194535No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCACTGAAGCGGTC12160.18708469428730118No Hit
CATTTACTGGGCTTCTATTCGGGAATCCGGGGTCCCTGACCGCTTCAGTG12050.1853923162962154No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12020.1849307586622829No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA11840.18216141285868798No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGC11780.181238297590823No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11620.17877665687651642No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTC11530.17739198397471895No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11360.17477649071576817No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11220.17262255509074992No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC11190.17216099745681746No Hit
GTTTATTACTGTCAGCAGTATTACAGCATCCCGTACACCTTTGGCCAGGG10980.16893009401929002No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGT10630.16354525495674435No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10630.16354525495674435No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCA10610.16323754986745603No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG10230.15739115317097788No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10130.15585262772453626No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC10130.15585262772453626No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10100.15539107009060377No Hit
CAGTAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTC9960.15323713446558548No Hit
CAGTAATAAACTCCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGT9950.15308328192094134No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9860.15169860901914387No Hit
GGTGTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCCACATCTT9840.15139090392985555No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA9790.15062164120663474No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT9780.1504677886619906No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9760.15016008357270225No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA9680.14892926321554895No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC9580.14739073776910733No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9530.14662147504588652No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT9430.1450829495994449No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9420.14492909705480075No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC9330.14354442415300328No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC9320.1433905716083591No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9290.14292901397442662No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9260.14246745634049415No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9230.14200589870656166No Hit
GCTCCAACAGTAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAG9170.14108278343869668No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9100.14000581562618755No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA8940.13754417491188095No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8820.13569794437615101No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8720.1341594189297094No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGGAGTTTATTACTGTCAGCAGTAT8720.1341594189297094No Hit
GTAAATGAGCACCCGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA8700.13385171384042105No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8670.13339015620648856No Hit
GTAATACTGCTGACAGTAATAAACTCCCACATCTTCAGCCTGCAGGCTGG8640.1329285985725561No Hit
CCACTGAAGCGGTCAGGGACCCCGGATTCCCGAATAGAAGCCCAGTAAAT8610.1324670409386236No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8600.13231318839397943No Hit
GGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCACTGAAG8580.1320054833046911No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATT8540.13139007312611448No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8510.13092851549218198No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8470.13031310531360535No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCACTGAAGCGGT8440.12985154767967286No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT8350.12846687477787538No Hit
CCCGTACACCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGG8340.1283130222332312No Hit
GTGTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCCACATCTTC8010.12323588825997388No Hit
CCTTGGTCCCCTGGCCAAAGGTGTACGGGATGCTGTAATACTGCTGACAG7990.12292818317068556No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7980.12277433062604139No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7960.12246662553675307No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG7950.12231277299210891No Hit
GGCTGAAGATGTGGGAGTTTATTACTGTCAGCAGTATTACAGCATCCCGT7850.12077424754566729No Hit
CAGTAAATGAGCACCCGAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGC7840.12062039500102312No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7820.1203126899117348No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAACAGTAA7810.12015883736709063No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATTCAGCTCCAACAGTAAGAACTACT7720.11877416446529317No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7700.11846645937600485No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7640.11754334410813988No Hit
GTACGGGATGCTGTAATACTGCTGACAGTAATAAACTCCCACATCTTCAG7560.11631252375098658No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA7390.11369703049203582No Hit
GTGTTTTATTCAGCTCCAACAGTAAGAACTACTTAGCTTGGTACCAGCAG7350.11308162031345918No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA7330.11277391522417086No Hit
GGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCG7210.11092768468844091No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7190.11061997959915257No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT7140.10985071687593176No Hit
GGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGA7080.10892760160806679No Hit
GCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTC7070.10877374906342263No Hit
AAGTAGTTCTTACTGTTGGAGCTGAATAAAACACTCTGGCTGGACTTGCA7060.10861989651877847No Hit
CCGTACACCTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGC7000.10769678125091349No Hit
CCCGAATAGAAGCCCAGTAAATGAGCACCCGAGGAGGCTGTCCTGGTTTC6950.10692751852769268No Hit
GCCTCCTCGGGTGCTCATTTACTGGGCTTCTATTCGGGAATCCGGGGTCC6940.10677366598304854No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT6900.10615825580447188No Hit
GCTAAGTAGTTCTTACTGTTGGAGCTGAATAAAACACTCTGGCTGGACTT6890.10600440325982771No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTGGGCTT6800.10461973035803027No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTCGGGTGCTCATTTACTG6740.10369661509016528No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG6720.10338891000087695No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC6710.1032350574562328No Hit
TCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATG6670.10261964727765614No Hit
GTATCAACGCAGAGTACGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGA6650.10231194218836782No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC6640.10215808964372365No Hit
GTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCAGAGATCCAGA6560.10092726928657036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)