FastQCFastQC Report
Wed 8 May 2024
EGAF00004692118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252229
Total Bases17.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34641.3733551653457772No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC16210.6426699546840371No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT12390.49122027998366563No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATG9810.38893227979336237No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG9530.37783125651689536No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA8460.33540948899611067No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA7910.31360390756019335No Hit
GGTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCC7470.2961594424114594No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC7160.28386902378394235No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT7120.28228316331587566No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG6780.2688033493373086No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG6200.24580837255034116No Hit
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAAC5780.22915683763564065No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCA5590.22162400041232372No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCA5380.21329823295497347No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTA5330.21131590736989006No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG5320.21091944225287337No Hit
GTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAG5260.2085406515507733No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC5240.20774772131673994No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA5170.20497246549762319No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC5020.199025488742373No Hit
ATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTA4820.1910961864020394No Hit
ACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTA4810.19069972128502274No Hit
CCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTA4810.19069972128502274No Hit
ATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCC4490.17801283754048902No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAG4190.16611888402998862No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC4190.16611888402998862No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC4170.16532595379595527No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAG4100.1625506979768385No Hit
GCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCT4000.15858604680667174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.15700018633860502No Hit
GGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG3870.1534320002854549No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.1530355351684382No Hit
TCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTG3780.14986381423230477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.14907088399827143No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTC3510.13915925607285443No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3420.13559107001970433No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT3370.13360874443462092No Hit
CCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGG3370.13360874443462092No Hit
TATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCA3240.12845469791340408No Hit
GGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCT3180.12607590721130402No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCC3170.12567944209428733No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.12488651186025397No Hit
CCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGC3090.12250772115815389No Hit
GTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACT3090.12250772115815389No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.1205253955730705No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2920.11576781416887036No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT2880.11418195370080364No Hit
GGTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGC2830.11219962811572025No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT2810.11140669788168689No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.1070455815945034No Hit
ATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGG2660.10545972112643669No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.10308093042433661No Hit
CCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCA2570.10189153507328658No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT2570.10189153507328658No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)