FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235342
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT5380.22860347919198445No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC5370.2281785656618878No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT5290.2247792574211148No Hit
GTATAACAGTGTCTCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5200.22095503565024519No Hit
CTGTTATACTGTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC4780.2031086673861869No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG4370.1856872126522253No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA4170.17718894205029276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3670.1559432655454615No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT3410.14489551376294924No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3380.14362077317265937No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3330.14149620552217623No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA3050.12959862667947072No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2960.1257744049086011No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC2900.12322492372802134No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA2860.12152526960763485No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2830.12025052901734497No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCG2820.11982561548724835No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC2730.11600139371637871No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2640.1121771719455091No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG2540.10792803664454284No Hit
CCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTA2540.10792803664454284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.10792803664454284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.10707820958434959No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2470.10495364193386644No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA2460.10452872840376982No Hit
GGTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCC2450.1041038148736732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.10367890134357657No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2420.10282907428338334No Hit
AGTCAGGACATTAGCAAGTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAACCTCCTGATCTATG2400.10197924722319007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGAT100.008033909139.0115470
TCTGAGT408.178321E-569.5057770
TGTCAGC200.0043882458.95481560
AGTTGAG250.005029677456.95313369
TCGTATG950.051.63154240
AAGTACG200.00985536448.051181
ATGCCGT1050.047.9632444
GAGACGT1150.047.47589524
CGTATGC1050.047.0975241
ACGTAGA1200.045.93832427
TTATACA1200.045.3623852
GACGTAG1250.043.93062226
CCCACGA1250.043.55714419
GTATGCC1150.043.29640642
GCCGTCT1100.042.81952746
AGACGTA1300.042.096625
TAGAGGA1350.041.3321530
CCGTCTT1150.041.1358447
CGTAGAG1350.040.99284728
ATCTCGT759.804353E-1040.87391337
Produced by FastQC (version 0.11.5)