FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709319
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT93690.5481130204484943No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC87620.5126018022382013No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC67660.39583015224191626No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62030.3628930585806394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55870.32685531489441116No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGGGTCACCATCACTTGCCG54980.3216485629657191No Hit
GTCTGCATCTGTGGGAGACAGGGTCACCATCACTTGCCGGGCCAGTCAGA54720.3201274893685731No Hit
ATATAGTACTTTTTCCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT53470.3128146355361404No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC51860.3033956797999671No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG51120.29906647033116696No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTACAACTTATTACTGCCAGCAATATAGTACTTTTTCCAGGACGTT50730.29678485993544795No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50110.29315768443456136No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49760.29111008536148025No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTACAACTTA48230.28215915227058264No Hit
GTACTATATTGCTGGCAGTAATAAGTTGTAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC47390.27724491449518784No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC45800.2679429644203335No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG44670.2613321445558143No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44040.2576464662242683No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43400.25390228506206275No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC43050.25185468598898153No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42910.2510356463597491No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41770.2443663236645705No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40840.2389255604132406No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40200.23518137925103508No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39440.230735164120916No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT39350.23020863864498084No Hit
GAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGACGCCTC39090.22868756504783486No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT38300.2240658414257374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC37020.21657747910132633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36980.2163434677786885No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC36770.2151149083348398No Hit
ATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA35970.2104346818820829No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35770.20926462526889364No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31730.1856294816824712No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC31570.18469343639191982No Hit
ACTTATTACTGCCAGCAATATAGTACTTTTTCCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA30910.1808322495683954No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29930.17509897216376813No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29780.17422142970387622No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29750.17404592121189782No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT29440.17223233346145453No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28020.163924931507811No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27440.16053176732956223No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27390.16023925317626495No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27260.15947871637769195No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAA27110.15860117391780001No Hit
CAATAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA26970.15778213428856755No Hit
ATATTATTGTGCGAAACGACCAATCTATGGCACCAACTGGGGGTACTTTAACTACTGGGGCCAGGGAGCCCTGGT26840.15702159748999456No Hit
GATCAGGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGC26360.1542134616183404No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCGTCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG25890.15146382857734572No Hit
GTGGGAGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAAA25890.15146382857734572No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA25880.15140532574668625No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTATTA25880.15140532574668625No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25810.15099580593207004No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25350.14830467572173478No Hit
GATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA25300.1480121615684375No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25210.14748563609250234No Hit
GTTATTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA25120.1469591106165672No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24720.1446189973901887No Hit
GCCCCTAACCTCCTGATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT24550.14362444926897788No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTA24440.1429809181317238No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24120.14110882755062104No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23790.1391782341388588No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCAT23650.13835919450962633No Hit
TCACCAGCCTGCAGCCTGATGATTTTACAACTTATTACTGCCAGCAATAT23520.13759865771105334No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC23430.1370721322351182No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23240.1359605784525884No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23170.1355510586379722No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22970.13438100202478295No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22920.13408848787148567No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22830.13356196239555052No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA22810.1334449567342316No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC22520.13174837464510722No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22410.13110484350785315No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22000.12870622745081522No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21990.12864772462015575No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC21780.12741916517630705No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21620.12648311988575567No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC21580.12624910856311783No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC21560.1261321029017989No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21560.1261321029017989No Hit
GTATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT21480.12566408025652323No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGA21290.12455252647399345No Hit
GGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT21170.1238504925060799No Hit
GTAATAAGTTGTAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG21140.12367498401410153No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20840.12191989909431768No Hit
GCCCTATATTATTGTGCGAAACGACCAATCTATGGCACCAACTGGGGGTACTTTAACTACTGGGGCCAGGGAGCC20780.1215688821103609No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20780.1215688821103609No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20760.12145187644904198No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20600.12051583115849061No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT20590.12045732832783114No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20440.11957978586793923No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG20390.1192872717146419No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20390.1192872717146419No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20290.1187022434080473No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20250.11846823208540944No Hit
ATATTGCTGGCAGTAATAAGTTGTAAAATCATCAGGCTGCAGGCTGGTGA20100.11759068962551754No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19950.1167131471656256No Hit
TCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC19920.11653763867364722No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19880.11630362735100938No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC19780.11571859904441477No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATACCATGAGCTGGGTCCGCCAGGCTCCAGGGAGGGGG19760.11560159338309584No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG19670.1150750679071607No Hit
TTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCTCCCACAGA19470.11390501129397146No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19420.11361249714067416No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19410.11355399431001469No Hit
CCCCTAACCTCCTGATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCA19330.113085971664739No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18840.11021933296242539No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18820.11010232730110647No Hit
CCTTGGTCCCTTGGCCGAACGTCCTGGAAAAAGTACTATATTGCTGGCAGTAATAAGTTGTAAAATCATCAGG18450.10793772256670639No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18010.10536359801769009No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG17970.10512958669505225No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17620.10308198762197109No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC17530.10255546214603595No Hit
CTTCCACCCTGTCTGCATCTGTGGGAGACAGGGTCACCATCACTTGCCGG17470.10220444516207917No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17430.10197043383944132No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCTGGA17310.10126839987152779No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC17240.10085888005691154No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG17140.10027385175031694No Hit
ATAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA17130.10021534891965747No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17110.10009834325833855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC200.007735538351.1020319
CGGACTA200.00774090851.0930679
TCGTATG1250.049.7139240
GTGGTAT2350.049.292791
CGTATGC1300.047.86997241
ATCTCGT1400.044.20525437
ACGAGAC1550.039.575622
ATGCCGT1600.039.02644344
GTATCAA27750.037.56911
CCGTCTT1750.035.8307647
CATCTCG1700.034.35650636
CTTATAC1900.034.0700151
TAGGACC502.0058898E-434.0650334
TATACAC2300.034.0650333
TACATCT1750.033.30512234
TATCAAC31000.033.2958222
TCCGAGC1800.032.1697113
AATGGTA8550.031.9073470
ATCAACG32350.031.9063553
TCAACGC32200.031.8434014
Produced by FastQC (version 0.11.5)