FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709319
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT93060.5444273421169483No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC75430.44128685166431775No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC65850.3852411398925537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62220.36400461236316917No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53780.3146282232865837No Hit
ATATAGTACTTTTTCCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT52850.3091874600352538No Hit
GTCTGCATCTGTGGGAGACAGGGTCACCATCACTTGCCGGGCCAGTCAGA52550.30743237511546995No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50950.2980719222099561No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49920.2920461306520316No Hit
GTACTATATTGCTGGCAGTAATAAGTTGTAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC49010.28672237306202064No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46640.27285720219572823No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC46590.27256468804243095No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT46520.2721551682278147No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTACAACTTA46060.2694640380174795No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTACAACTTATTACTGCCAGCAATATAGTACTTTTTCCAGGACGTT44540.26057160775724136No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43490.2544288105379979No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGGGTCACCATCACTTGCCG43270.25314174826348973No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC41480.24266974157544616No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40190.2351228764203756No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40020.23412832829916475No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39490.23102767827421328No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38230.22365632161112114No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37710.22061417441682918No Hit
GAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGACGCCTC37510.2194441178036399No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG36800.21529041682681818No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT36360.21271629227780187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36190.21172174415659104No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35870.20984965357548824No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC35450.20739253468779087No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT35350.20680750638119627No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA35180.20581295825998538No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34380.2011327318072285No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33530.19615999120117428No Hit
ATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA33360.19516544307996342No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC32800.19188928456303359No Hit
GTGGGAGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAAA32720.1914212619177579No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31760.1858049901744496No Hit
ACTTATTACTGCCAGCAATATAGTACTTTTTCCAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA31550.1845764307306009No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30950.1810662608910332No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA28960.16942419758980037No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28460.1664990560568273No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC28190.16491947962902184No Hit
ATATTATTGTGCGAAACGACCAATCTATGGCACCAACTGGGGGTACTTTAACTACTGGGGCCAGGGAGCCCTGGT27020.15807464844186486No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27000.15795764278054594No Hit
GTTATTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA26320.15397945029570256No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26030.1522828682065782No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT25800.15093730310141057No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25620.14988425214954026No Hit
CAATAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA25580.1496502408269024No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25340.14824617289107533No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25180.14731012760052395No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTATTA24920.14578905400337794No Hit
GATCAGGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGC24860.14543803701942118No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24850.14537953418876173No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTGGGAGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATAA24550.14362444926897788No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24420.14286391247040486No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24360.14251289548644813No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGA24240.1418108615185346No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24180.1414598445345778No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24000.1404067935827075No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC23990.14034829075204805No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23940.14005577659875074No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23140.1353755501459938No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCGTCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG22980.13443950485544243No Hit
GATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA22890.13391297937950727No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC22600.1322163972903829No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22490.13157286615312883No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG22440.1312803519998315No Hit
GTAATAAGTTGTAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG22170.12970077557202606No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22050.1289987416041125No Hit
GCCCCTAACCTCCTGATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT21900.1281211991442206No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21730.12712665102300974No Hit
TCACCAGCCTGCAGCCTGATGATTTTACAACTTATTACTGCCAGCAATAT21650.12665862837773406No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21640.1266001255470746No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTA21610.12642461705509622No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCAT21490.12572258308718268No Hit
GCCCTATATTATTGTGCGAAACGACCAATCTATGGCACCAACTGGGGGTACTTTAACTACTGGGGCCAGGGAGCC21410.12525456044190697No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21190.12396749816739883No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21000.12285594438486906No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG20990.12279744155420959No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC20920.12238792173959337No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20810.1217443906023393No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20590.12045732832783114No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC20470.1197552943599176No Hit
GGACTGAGCAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT20440.11957978586793923No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT20380.11922876888398246No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC20140.11782470094815536No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19880.11630362735100938No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19730.11542608489111744No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19640.11489955941518232No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19550.11437303393924715No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19350.11320297732605791No Hit
ATATTGCTGGCAGTAATAAGTTGTAAAATCATCAGGCTGCAGGCTGGTGA19040.11138938957561463No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18760.1097513103171497No Hit
TTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCTCCCACAGA18750.10969280748649023No Hit
GTATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT18720.10951729899451185No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18720.10951729899451185No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18620.10893227068791723No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACT18490.10817173388934424No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18430.10782071690538747No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18420.107762214074728No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18310.10711868293747392No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATACCATGAGCTGGGTCCGCCAGGCTCCAGGGAGGGGG18020.10542210084834955No Hit
TCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC18020.10542210084834955No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17940.10495407820307387No Hit
CCTTGGTCCCTTGGCCGAACGTCCTGGAAAAAGTACTATATTGCTGGCAGTAATAAGTTGTAAAATCATCAGG17740.10378402158988463No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17710.10360851309790624No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC17680.10343300460592786No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17420.10191193100878186No Hit
CCCCTAACCTCCTGATCTATGACGCCTCCAGTTTGGAAAGTGGGGTCCCA17400.10179492534746293No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17370.10161941685548455No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG17340.10144390836350618No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17340.10144390836350618No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17180.10050786307295477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)