FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669923
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATCCCCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT220511.3204800460859571No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGGGTGAATTCTGTC209271.25317155341893No Hit
GTTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA111980.6705698406453471No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99940.5984707079308448No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC76000.45511080451014807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC67460.4039707220033498No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC65030.3894191528591438No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62760.3758257117244328No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT57150.34223134839151265No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54810.3282187262526476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52840.31642177513573977No Hit
GGTATTAACAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA52320.3133078591048809No Hit
GAATTCACCCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAT49360.2955824909292225No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG47010.281509985789764No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTGTTAATACCTGGTTGGCCTGGTATCAGCAGAAA46380.2777373567523772No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA46040.2757013347322002No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG45970.27528215372804615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45640.2733060147084626No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT45000.2694735026704824No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC44940.26911420466692176No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT44840.2685153746609874No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44410.2659404056354694No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43760.26204801059689575No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42770.2561195935381452No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT41720.24983187847583394No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40940.24516100442954555No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39680.23761574635477203No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT39490.23647796934349666No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37860.22671704024676587No Hit
GGGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA37330.22354324121531352No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG37120.22228569820285127No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA37110.22222581520225781No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36090.2161177491417269No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35630.21336313111442862No Hit
GTAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGG35520.2127044181079008No Hit
CAGTATAATAGTTATCCCCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT35300.21138699209484507No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC33330.19959004097793728No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32170.1926436129090982No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC31410.1880925048639967No Hit
CTGTTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTACAAGGCGTCTA31290.18737390885687544No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31110.18629601484619351No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTGTTAATAC29980.17952923577913474No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29630.17743333075836432No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29000.17366070172097756No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC28400.17006772168537113No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27990.16761251866104007No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27780.16635497564857782No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27080.16216316560703697No Hit
ACCTTGATGGGACCCCAGTTGCTAAAGTAGACGCCTTGTAGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTG25600.15330048151920778No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25470.15252200251149303No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25380.1519830555061521No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25170.15072551249368984No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC24630.14749183046164405No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG24600.14731218145986372No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTACAAGGCGTCTACTTTAGCAACTGGGGTCCCAT24600.14731218145986372No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGGGGATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG24600.14731218145986372No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24450.14641393645096212No Hit
GGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGTTGCTAAAGTAGACGCCTTGTAGAT24410.14617440444858837No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23960.14347966942188353No Hit
GTATTAACAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA23860.14288083941594912No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23780.1424017754112016No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC23680.1418029454052672No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23440.1403657533910246No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23190.1388686783761886No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC23040.137970433367287No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22760.13629370935067064No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTATCCCCTCACTTT22580.13521581533998872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22230.1331199103192183No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22150.1326408463144708No Hit
GCTGATGGTGAGGGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGTTGCTAAAGTAGA22000.13174260130556917No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21790.13048505829310691No Hit
GCCCCTAAGTTCCTGATCTACAAGGCGTCTACTTTAGCAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT21770.13036529229192004No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG21730.13012576028954628No Hit
ACTTATTACTGCCAACAGTATAATAGTTATCCCCTCACTTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA21460.1285089192735234No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21380.12802985526877586No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21150.12665254625512673No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20810.12461652423494975No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20760.12431710923198255No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20690.12389792822782845No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20420.12228108721180557No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGGGTGAATTCTGTCCCAGATCCACTGCCG20410.12222120421121213No Hit
GACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT20350.1218619062076515No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20340.12180202320705805No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20300.12156249120468428No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20080.1202450651916286No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGTTGCTAAAGT19770.11838869217323195No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACCCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG19710.1180293941696713No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19650.11767009616611065No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19640.1176102131655172No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19560.11713114916076968No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19260.11533465914296648No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTATTAA18740.11222074311210756No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18730.11216086011151412No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18720.1121009771109207No Hit
GATCTACAAGGCGTCTACTTTAGCAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC18660.11174167910736005No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18330.10976554008777652No Hit
GTCAGACTGTTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTACAAGG18290.10952600808540275No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18220.10910682708124865No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCC18130.1085678800759077No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTC18120.10850799707531425No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC17850.10689115605929135No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17690.1059330280497963No Hit
ACTTTAGCAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACCCTCACCATCAGCAGC17680.10587314504920287No Hit
GTGTATTACTGTGCGAGAGGTTACATTGATAGTAGGGGTTTTTACTACTC17600.10539408104445534No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC17430.10437607003436686No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17320.103717357027839No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16770.10042379199519978No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16760.10036390899460634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC200.007132479552.16187746
GTGGTAT3400.045.0538251
GCTTGTA1350.040.3444941
GTATCAA32400.040.239431
AGGGACC50400.035.1326431
GAGGGAC50600.035.0223230
CGGAGGG51150.034.6791228
TATCAAC37300.034.6566432
AATGGTA4550.034.3972170
TTTCGGC52100.034.15802822
GCGGAGG52100.034.0242927
CGGCGGA52200.033.9917625
TCGGCGG52300.033.9084624
TTCGGCG52600.033.77572623
GGCGGAG52300.033.61528826
ATCAGGC47150.033.41367736
ATCAACG38950.033.3581963
ACTTTCG54150.033.30275720
GGACCAA53700.033.26933733
CTTTCGG54100.033.20874421
Produced by FastQC (version 0.11.5)