FastQCFastQC Report
Wed 8 May 2024
EGAF00004692127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739967
Total Bases54.2 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17190.23230765696308076No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAG14970.20230631906557997No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC12130.16392622914265095No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11500.15541233595552234No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10960.1481147132236978No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTT10550.1425739255939792No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10360.14000624352167057No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATAATTCTGTCTCCT10250.13851969074296558No Hit
ATAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA10080.13622229099405783No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT9810.13257347962814559No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9770.13203291498134376No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9310.12581642154312286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.12460015108781879No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9050.12230275133891105No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8680.11730252835599425No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC8580.11595111673898971No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA8520.11514026976878698No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGCAATTACTACTGGGGCTTCATCCG8430.11392399931348289No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATA8170.11041032910927108No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8150.11014004678587018No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG8150.11014004678587018No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8000.10811292936036337No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT7990.10797778819866292No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGTTCACAT7870.10635609425825746No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA7830.10581552961145566No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7510.1014910124370411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)