FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739967
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25330.3423125625872505No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC14880.20109004861027588No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAGAGTGCCGCCGGTTCTATGTGAACTG13460.18190000364881137No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11220.15162838342790963No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG10520.14216850210887783No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10290.13906025538976738No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTT9080.12270817482401243No Hit
ATAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA8820.11919450461980062No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC8760.11838365764959789No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8650.1168971048708929No Hit
CTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATAATTCTGTCTCCTGGTACCAACAACACCCAGGCAAAGC8520.11514026976878698No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGCAATTACTACTGGGGCTTCATCCG8470.11446456396028472No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8160.11027518794757063No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG8070.10905891749226654No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8020.10838321168376427No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7750.10473440031785201No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACGTTGGTGGTTATAATTCTGTCTCCTGGTACCAACAACA7620.1029775652157461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG200.00813287450.4557235
GTATCAA22950.034.7646031
GGTATCA10800.034.2878721
ACCGGAT400.002938919533.6371463
GTGAGCG4350.033.5833970
TACGGAT3900.030.6477870
TATCAAC26650.029.6692982
ATCAACG27450.028.9193693
TCAACGC27850.028.6247924
CAACGCA28050.028.3007765
AACGCAG28300.028.0507686
AGAGTAC28350.027.76399811
CGCAGAG28800.027.4488358
ATACGTA500.00875017226.9097211
CAGAGTA29650.026.54668810
GCAGAGT29800.026.5277359
GACAACG550.0096534926.37260859
ACGCAGA30350.026.1578397
AGTACGG20650.024.11776413
GAGTACG21050.023.49166512
Produced by FastQC (version 0.11.5)