FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545064
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT58160.37642453645933116No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC52940.3426395282007736No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG51950.3362320266344954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51920.3360378599203657No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC49720.32179896755085874No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATACCACTTGTTG46360.3000522955683389No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG44680.289178959577079No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43700.28283618024884405No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC42250.27345145573257806No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT42040.2720922887336706No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC41040.2656200649293492No Hit
GATTATTACTGCTGCTCACATGGAGGCTACAACAAGTGGTATGTCTTCGG40500.2621250640750157No Hit
ACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATACCACTTGTTGT39490.2555881180326511No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATACCACTTGTTGTAGCCTCCATGTGAG37620.2434850595185701No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC36190.23422977947839055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36090.23358255709795842No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC35950.23267644576535343No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35540.23002283400558166No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGC32660.21138282944913608No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTC31800.20581671697741968No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG31430.2034219941698208No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31120.20141560479048115No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30730.19889143750679586No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCACAT30610.1981147706502773No Hit
CCCCTGAGGGCCGCTGACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAGGAGA28780.18627060108836915No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG28730.1859469898981531No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27490.17792143238079458No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTA27130.17559143181123887No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA27090.17533254285906605No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC26080.16879559681670145No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25880.16750115205583718No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25860.16737170757975076No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25480.16491226253410862No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25390.1643297623917197No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT24760.16025226139499724No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24350.15759864963522546No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT24260.15701614949283654No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23320.15093225911677446No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA23250.15047920345047197No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23230.15034975897438552No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC22900.14821392511895948No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCG22270.144136424122237No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC21940.14200059026681094No Hit
GGATGAGGCTGATTATTACTGCTGCTCACATGGAGGCTACAACAAGTGGTATGTCTTCGGAACTGGGACCAGGGT21810.14115920117224917No Hit
CTGTTGGTACCAGGAGACATAGTTATAAGCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA21760.1408355899820331No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG21160.13695225569944028No Hit
GTTGTAGCCTCCATGTGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGC20440.1322922545603289No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20320.13151558770381033No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20260.13112725427555105No Hit
GTAATAAAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA20220.1308683653233782No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20140.1303505874190325No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA20040.12970336503860036No Hit
GCTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTCAG19630.12704975327882856No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19440.1258200307560075No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19250.12459030823318647No Hit
GGCTACAACAAGTGGTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA19100.12361947466253825No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT18620.12051280723646399No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18300.11844169561908115No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG18300.11844169561908115No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18260.11818280666690831No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18240.11805336219082185No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC18240.11805336219082185No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18180.11766502876256257No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18120.1172766953343033No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGC17720.11468780581257475No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT17690.11449363909844511No Hit
GTACTGTTGGTACCAGGAGACATAGTTATAAGCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA17630.11410530567018583No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTG17570.11371697224192653No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT17510.11332863881366725No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17470.11306974986149441No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG17380.1124872497191055No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT17370.11242252748106228No Hit
GGGTACTGTTGGTACCAGGAGACATAGTTATAAGCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGA17360.11235780524301907No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17340.11222836076693263No Hit
GGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC16950.1097041934832473No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16740.1083450264843398No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16560.10718002619956196No Hit
ACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTCAGCGGCCCTCAGGGGTCCCTGATCGCTT16520.1069211372473891No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16180.10472058115391983No Hit
GTTATAAGCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG16040.10381446982131486No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16020.10368502534522842No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTCAGCGGCCCTCAGGGGTCCCTGATCG15990.10349085863109878No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15970.10336141415501236No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15800.10226113610827771No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15760.10200224715610487No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC15640.10122558029958631No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15500.1003194689669813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)