FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439969
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGCTCACCTCACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT58500.40625874584800087No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54080.37556364060615194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48770.3386878467522565No Hit
GTATTACTGTCAGCAGTATAGTAGCTCACCTCACACTTTCGGCGGAGGGA42030.2918812835554099No Hit
CTACTATACTGCTGACAGTAATACGCTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGTGAGAAGTATGTC41800.29028402694780236No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37900.26320011055793563No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTGGCAGCACTTACTTAGCCTGGTACC35500.2465330850872484No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35050.24340801781149454No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34040.23639397792591366No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33290.23118553246632392No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC33140.23014384337440597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32920.22861603270625963No Hit
CAGTAATACGCTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGTGAGAAGTATGTCCCAGACCCACTGCCA31780.2206991956076832No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29880.20750446711005582No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT29080.2019487919531601No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28950.20104599474016455No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28590.19854594091956146No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27270.18937907691068348No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27060.18792071218199838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26440.18361506393540417No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24500.170142551679932No Hit
GGGACATACTTCTCACTCACCATCAGCAGGCTGGAGCCTGAAGATTTTGC24330.16896197070909164No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23750.16493410622034224No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23730.16479521434141986No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG23040.16000344451859727No Hit
TACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA22980.1595867688818301No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22250.15451721530116272No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTG22060.1531977424514No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21600.15000322923618495No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATGCAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTGGCAG21580.14986433735726257No Hit
GTATGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAG21570.14979489141780133No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20710.14382254062413843No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20540.1426419596532981No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGTAAGTGCTGCCAACACTCTGA20500.1423641758954533No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGATGCAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTGGCAGCA20490.1422947299559921No Hit
GTCTTTGTCTCCAGGGGATGCAGCCACCCTCTCCTGTAGGGCCAGTCAGA19440.13500290631256645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19400.13472512255472166No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19100.13264174437088577No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18310.12715551515345122No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18170.12618327200099447No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18000.12500269103015413No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTT17800.12361377224093019No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT17650.12257208314901225No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17580.12208596157278387No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17350.12048870496517633No Hit
GTCTGGGACATACTTCTCACTCACCATCAGCAGGCTGGAGCCTGAAGATT17130.11896089429703001No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT16960.11778031332618966No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16850.1170164079921165No Hit
CTACAGGAGAGGGTGGCTGCATCCCCTGGAGACAAAGACAGGGTGCCTGG16530.11479413792935819No Hit
GTCAGAGTGTTGGCAGCACTTACTTAGCCTGGTACCAGCACAAACCTGGC16430.11409967853474624No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16190.11243297598767751No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16140.11208574629037152No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15660.10875234119623409No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15580.1081967736805445No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15490.10757176022539373No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15370.10673840895185938No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA15230.10576616579940264No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14890.10340500385772194No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14890.10340500385772194No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14670.1018771931895756No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14530.10090495003711886No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA14440.1002799365819681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)