FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439969
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGCTCACCTCACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT55530.38563330182802547No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54590.37910538351867296No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41540.28847843252181127No Hit
CTACTATACTGCTGACAGTAATACGCTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGTGAGAAGTATGTC41400.2875061893693545No Hit
GTATTACTGTCAGCAGTATAGTAGCTCACCTCACACTTTCGGCGGAGGGA40980.2845894599119842No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37460.26014448922164296No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35770.24840812545270072No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34790.24160242338550345No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTGGCAGCACTTACTTAGCCTGGTACC33320.2313938702847075No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC33080.22972716773763882No Hit
CAGTAATACGCTGCAAAATCTTCAGGCTCCAGCCTGCTGATGGTGAGTGAGAAGTATGTCCCAGACCCACTGCCA32380.22486595197535503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30630.2127129125696456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30150.20937950747550813No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30040.20861560214143499No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29510.2049349673499916No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT28730.1995181840720182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26790.18604567181654605No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25670.17826772659689202No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24570.17062867325616038No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23390.16243405239973915No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23360.16222571458135557No Hit
GGGACATACTTCTCACTCACCATCAGCAGGCTGGAGCCTGAAGATTTTGC23220.16125347142889881No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22990.1596562148212913No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22950.15937843106344649No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22750.15798951227422256No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22590.15687837724284343No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21540.14958655359941778No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21410.1486837563864222No Hit
GTATGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAG21370.1484059726285774No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG21360.14833652668911623No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG20900.14514201347390118No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATGCAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTGGCAG20780.1443086622003668No Hit
TACTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA20700.14375309468467726No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGATGCAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTGGCAGCA20470.14215583807706972No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20330.14118359492461297No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGTAAGTGCTGCCAACACTCTGA19180.13319731188657533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18890.13118337964220064No Hit
GTCTTTGTCTCCAGGGGATGCAGCCACCCTCTCCTGTAGGGCCAGTCAGA18690.1297944608529767No Hit
GTCTGGGACATACTTCTCACTCACCATCAGCAGGCTGGAGCCTGAAGATT18480.12833609612429156No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18330.12729440703237363No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTG18160.12611382606153326No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17160.1191692321154136No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16870.11715529987103888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16800.11666917829481051No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTT16750.11632194859750453No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16490.11451635417151342No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16170.11229408410875512No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT16100.11180796253252674No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT16080.11166907065360435No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15990.11104405719845357No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15680.10889123307515648No Hit
CTACAGGAGAGGGTGGCTGCATCCCCTGGAGACAAAGACAGGGTGCCTGG15340.10653007113347579No Hit
GGTTTGTGCTGGTACCAGGCTAAGTAAGTGCTGCCAACACTCTGACTGGCCCTACAGGAGAGGGTGGCTGCATCC15280.1061133954967086No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA15260.10597450361778621No Hit
GTCAGAGTGTTGGCAGCACTTACTTAGCCTGGTACCAGCACAAACCTGGC15090.10479392264694587No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14930.10368278761556672No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC14930.10368278761556672No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC14870.10326611197879955No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14580.10125217973442482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)