FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514709
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49880.3293041765778113No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43240.28546737360113394No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42440.2801858310738234No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41180.27186740159330935No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40680.26856643751374026No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC38560.2545703498163674No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38250.25252375208703454No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35920.23714125947624262No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33950.22413546100274045No Hit
GACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCGCTTCTGTCCCACGCCTGACAGTAATAGT32440.21416654948244185No Hit
CCCCTGTTCTGGTCATCTATCAACATAACAAGCGGCCCTCAGGGATCTCT31010.20472579221487425No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG30520.2014908474168966No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC29550.19508697710253256No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28680.18934329960408236No Hit
GACTATTACTGTCAGGCGTGGGACAGAAGCGCTGGAGGAGTCTTCGGAACTGGGACCAAAGTCACCGTCCTAGGT28570.18861708750657719No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27800.1835336028240408No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26960.17798798317036474No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26350.17396080699329047No Hit
CAGCCACTCTGACCATCAGTGAGACCCAGACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAAGCG26020.17178217070077487No Hit
GCCTTGGGCTGACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCGCTTCTGTCCCACGCCTGA25670.1694714958450765No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25200.16636858961028156No Hit
CTATTACTGTCAGGCGTGGGACAGAAGCGCTGGAGGAGTCTTCGGAACTG25190.1663025703286902No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23800.15712589018748815No Hit
ACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCGCTTC23470.15494725389497258No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22760.1502598849019845No Hit
GGACAGAAGCGCTGGAGGAGTCTTCGGAACTGGGACCAAAGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA20680.1365278743309771No Hit
GAACAGGGGACTGGCCTGGCTTCTGCTGATACCAACATGCATATTTATTG20600.13599972007824604No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20460.13507545013596672No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20220.13349098737777354No Hit
GACCCAGACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAA19930.1315764282116235No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19780.13058613898775276No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19590.1293317726375165No Hit
ATGTATTACTGTGCGAGAGACCGGGATGACTACGGTGAATTCGACTACTGGGGCCAGGGAACCCTGGTCACCGT19370.12787934844250612No Hit
GACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAAGCGCTG19280.1272851749081837No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19160.1264929435290871No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCG18860.12451236508134567No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG18610.12286188304156112No Hit
GTAATAGTCAGCCTCATCCATAGTCTGGGTCTCACTGATGGTCAGAGTGG17960.11857062973812131No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17820.11764635979584198No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17670.11665607057197126No Hit
CCTATGACCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCGGGACAGACA17510.11559976206650914No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17180.11342112577399355No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG16830.1111104509182952No Hit
CTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAAGCGCTGGA16570.10939394959691927No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16560.10932793031532789No Hit
CCCTCAGTGTCCGTGTCCCCGGGACAGACAGCCAGCATCACCTGCTCTGG16390.10820560252827441No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16150.10662113977008125No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT15770.10411240706960875No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15740.1039143492248346No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15250.10067940442685691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)