FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514709
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47610.3143177996565677No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45890.3029624832228501No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43570.2876460098936495No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38410.25358006059249666No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37820.24968492297860514No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35960.23740533660260815No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34930.23060535059869586No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34840.23001117706437343No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31570.20842287198399165No Hit
CCCCTGTTCTGGTCATCTATCAACATAACAAGCGGCCCTCAGGGATCTCT31320.20677238994420713No Hit
GACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCGCTTCTGTCCCACGCCTGACAGTAATAGT29330.19363455290752216No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG28910.19086174308068415No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28540.18841902966180302No Hit
GACTATTACTGTCAGGCGTGGGACAGAAGCGCTGGAGGAGTCTTCGGAACTGGGACCAAAGTCACCGTCCTAGGT27440.18115690868675105No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27160.17930836880219236No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26040.17191420926395762No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25750.16999965009780757No Hit
GCCTTGGGCTGACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCGCTTCTGTCCCACGCCTGA25480.16821712949484027No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24200.1597666614511434No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24030.15864433366408992No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23480.15501327317656394No Hit
CAGCCACTCTGACCATCAGTGAGACCCAGACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAAGCG23480.15501327317656394No Hit
CTATTACTGTCAGGCGTGGGACAGAAGCGCTGGAGGAGTCTTCGGAACTG22360.14761911363832922No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21940.1448463038114912No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21860.14431814955876013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21150.13963078056577205No Hit
ACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCGCTTC20520.135471565825515No Hit
GTAATACATGGCCGTGTCCTCAGATCTCAGGCTGCTAATTTCCATGTACACTGTGCTCGTGGGCGTGTCCCTGGT19830.13091623539570968No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACTCCTCCAGCG19600.12939779191910789No Hit
GACCCAGACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAA19530.12893565694796824No Hit
GGACAGAAGCGCTGGAGGAGTCTTCGGAACTGGGACCAAAGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA18810.12418226867338875No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18790.12405023011020598No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18570.12259780591519559No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18410.12154149740973348No Hit
GAACAGGGGACTGGCCTGGCTTCTGCTGATACCAACATGCATATTTATTG18390.12140945884655072No Hit
ATGTATTACTGTGCGAGAGACCGGGATGACTACGGTGAATTCGACTACTGGGGCCAGGGAACCCTGGTCACCGT18180.1200230539331317No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18150.11982499608835756No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17270.114015299308316No Hit
GACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAAGCGCTG17170.11335510649240217No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16920.11170462445261763No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16610.1096580267232848No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16610.1096580267232848No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT15930.10516871557507085No Hit
CCTATGACCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCGGGACAGACA15930.10516871557507085No Hit
GTAATAGTCAGCCTCATCCATAGTCTGGGTCTCACTGATGGTCAGAGTGG15840.10457454204074842No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15760.10404638778801736No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15740.1039143492248346No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15620.10312211784573803No Hit
GAGTGGCTGTGTCCCCAGAGTTGGAGCCAGAGAATCGCTCAGAGATCCCT15520.10246192502982421No Hit
CCCTCAGTGTCCGTGTCCCCGGGACAGACAGCCAGCATCACCTGCTCTGG15460.10206580934027593No Hit
CTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAAGCGCTGGA15320.10114153939799657No Hit
GACCATCAGTGAGACCCAGACTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGAAGCGCTGGAGGAGT15210.10041532730049138No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15190.10028328873730862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)