FastQCFastQC Report
Wed 8 May 2024
EGAF00004692137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165488
Total Bases10.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT19941.2049212027458183No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT8800.5317606110412839No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.37948370878855264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.29669825002417094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.2840085081697767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.27796577395339844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.23627090786038865No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC3890.23506236101711303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.20122304940539495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.1915546746591898No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.19034612781591415No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.17705211253988204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.17644783911824422No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.17584356569660642No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA2570.15529826936092042No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.1522769022527313No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.1492555351445422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.14804698830126656No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.14744271487962873No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG2430.1468384414579909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.14260852750652614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2350.14200425408488834No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.13596151986851013No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG2220.13414869960359663No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.12689741854394276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.12387605143575366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.12266750459247801No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1920.11602049695446194No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.11360340326791066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.11058203615972155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10876921589480808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10876921589480808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.10816494247317025No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.10816494247317025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.10333075510006767No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCC1680.1015179348351542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)