FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092736
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCCCTCAGGCTGTCCATTTGCAAAAAAA30020.27472326344149No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29370.2687748916481199No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCA25330.23180347311702004No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24660.22567207449923862No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAACGATGTTA21770.19922469837179335No Hit
GTCTTGGGCTCAGGCCTGAATCGGTTCTTGAGTATCAAGAACATCCAGGA20840.19071395103666394No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20730.1897073035024013No Hit
CCCTTACTGCATACCCTTCTGACACAGTAATAGACAGCCGTGTCCTCGGC20550.18806006208269885No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19690.18018990863300927No Hit
GTCTATTACTGTGTCAGAAGGGTATGCAGTAAGGGTGAGTGCTTCTCGCA19640.17973234157198079No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18330.16774408457303502No Hit
ATGTTATTCCCAGTGCCATGGTCTGTCGCACAATAGTAGTCACTCTCATC18240.1669204638631838No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17250.1578606360548202No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17200.15740306899379172No Hit
GTACCAGCAGAGACCAGGGGAGGGGCCCCAATTTGTGATGCGGGTGGGCC16850.1542000995665925No Hit
CACCTGCACCCTGAGTAGCGGCTACAGTAATTATAGAGTCGACTGGTACC16540.151363183788216No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16500.15099713013939323No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT16220.14843475459763383No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16200.14825172777322243No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16100.14733659365116553No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16020.14660448635351997No Hit
ATAGTAGTCACTCTCATCCTCCTCCTGGATGTTCTTGATACTCAAGAACC15860.14514027175822888No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15570.1424863828042638No Hit
GTAATTATAGAGTCGACTGGTACCAGCAGAGACCAGGGGAGGGGCCCCAA15430.1412051950333841No Hit
GGATGAGAGTGACTACTATTGTGCGACAGACCATGGCACTGGGAATAACATCGTTTGGATATTCGGCGGAGGGAC15260.13964946702588732No Hit
GACCAGGGGAGGGGCCCCAATTTGTGATGCGGGTGGGCCTTGGTGGGATTGTGGGATCCAAGGGGGATGGCATTC15020.13745314513295068No Hit
GAATAACATCGTTTGGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT14660.13415866229354576No Hit
CTCCCAGCCTGTGGTGACTCAGCCACCTTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACC14380.13159628675178636No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGATTCACCTTG14040.1284848307367928No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG13890.12711212955370738No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13870.126929102729296No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13870.126929102729296No Hit
CTCTCATCCTCCTCCTGGATGTTCTTGATACTCAAGAACCGATTCAGGCC13810.12638002225606185No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13780.12610548201944477No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13710.12546488813400491No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13680.12519034789738784No Hit
CTACTATTGTGCGACAGACCATGGCACTGGGAATAACATCGTTTGGATATTCGGCGGAGGGACCAAGCTGACCGT13680.12519034789738784No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13600.1244582405997423No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13480.12336007965327397No Hit
GGCCTGAATCGGTTCTTGAGTATCAAGAACATCCAGGAGGAGGATGAGAG13210.12088921752372028No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13140.12024862363828041No Hit
GACTACTATTGTGCGACAGACCATGGCACTGGGAATAACATCGTTTGGAT13070.11960802975284057No Hit
GCGTAATACAGGTGATCAGTACTAAAAACCGATGAGACCCACTCCAGTCC12740.11658808715005271No Hit
CTGCTGGTACCAGTCGACTCTATAATTACTGTAGCCGCTACTCAGGGTGC12720.11640506032564131No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12700.11622203350122995No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12640.11567295302799578No Hit
CAATAGTAGTCACTCTCATCCTCCTCCTGGATGTTCTTGATACTCAAGAACCGATTCAGGCCTGAGCCCAAGA12390.11338511772285345No Hit
CCTCTGCTCCTCACCCTCCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCC12290.11246998360079653No Hit
GTGTGAGGTGCAGCTGGTGGAGTCGGGGGGGGGCCTGGTCAAGCCTGGAGGGTCCCTGAGACTCTCCTGTGAAGC12200.1116463628909453No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12150.11118879582991684No Hit
ATTCAGGCCTGAGCCCAAGACTGAGAATCGATCAGGAATGCCATCCCCCTTGGATCCCACAATCCCACCAAGGCC12140.11109728241771114No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12110.11082274218109406No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12070.1104566885322713No Hit
CAGTAAGGGTGAGTGCTTCTCGCAGGCGGGCTCCTATTACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGT11940.10926701417359728No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11670.10679615204404358No Hit
GTCCCTCTCCCAGCCTGTGGTGACTCAGCCACCTTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTG11640.10652161180742649No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11610.10624707157080941No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11500.1052404240365468No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11500.1052404240365468No Hit
CCTGCACCCTGAGTAGCGGCTACAGTAATTATAGAGTCGACTGGTACCAG11400.10432528991448986No Hit
GCCCAAGACTGAGAATCGATCAGGAATGCCATCCCCCTTGGATCCCACAA11330.10368469602905002No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11210.10258653508258171No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11180.10231199484596462No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11100.10157988754831908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)