FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558312
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG91301.6352863631804437No Hit
GTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC85631.5337302440212641No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT64371.152939575004657No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA61961.1097737465789739No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT58031.0393829973204947No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT43630.7814626946940063No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC40320.7221768473541675No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC37920.6791901302497528No Hit
GGTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCG37250.6671896717247704No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC33930.6077247130636634No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG33500.6000229262491223No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC33430.5987691470002436No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA32920.5896344696155554No Hit
GAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT32400.5803206809095989No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT32110.5751264525928155No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT30690.5496926449727034No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC25170.4508231956325495No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT25060.44885297109859723No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC24380.4366734012523464No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG23550.4218071615870696No Hit
GTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC22360.4004929143561306No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT22020.3944031294330052No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG21730.38920890111622175No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC21310.3816862256229492No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT20550.36807376520655116No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT19800.35464041611142155No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT19720.35320752554127444No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA19350.3465804066543438No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC19140.3428190689077075No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT18590.3329679462379458No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA18320.32813194056369915No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT17930.32114659903423176No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCA17810.31899726317901106No Hit
TATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCC17280.3095043631517861No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA16820.30126524237343993No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG16110.2885483385633839No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC15790.2828167762827953No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA15490.27744343664474347No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA15450.2767269913596698No Hit
CCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT14590.2613234177305879No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG14500.25971141583917234No Hit
CCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA14270.2555918554499993No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC14210.25451718752238894No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACCCCGTA14210.25451718752238894No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT14100.2525469629884366No Hit
ATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCC13890.24878562524180028No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC13680.24502428749516397No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG13260.23750161200189143No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT13010.23302382897018156No Hit
GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT12680.22711315536832452No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA12360.22138159308773586No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC12180.21815758930490478No Hit
GGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGT11960.21421714023700011No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG11950.2140380289157317No Hit
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA11800.2113513590967058No Hit
CTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTG11390.2040077949247016No Hit
CCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTAC10820.1937984496124031No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA10080.18054421183854188No Hit
CCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG9990.17893220994712633No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG9750.17463353823668487No Hit
GTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC9660.17302153634526932No Hit
GTATCAACGCAGAGTACGGGTGGCGGCGACGACCCATTCGAACGTCTGCC9600.17194686841765894No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA9470.16961842124116983No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGG9210.16496152688819155No Hit
TTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC9180.16442419292438637No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCG9120.163349524996776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.15833440800126095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.15582684950350342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.15421484761208787No Hit
CCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGT8540.1529610683632091No Hit
TCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC8470.15170728911433035No Hit
CATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT8430.15099084382925676No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC8180.1465130607975469No Hit
GAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGC7670.13737838341285877No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA7470.13379615698749087No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA7090.12698992677929186No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCA7040.1260943701729499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.12501970224533954No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT6940.12430325696026594No Hit
GGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC6910.12376592299646076No Hit
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA6860.1228703663901188No Hit
CTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTC6850.12269125506885038No Hit
GTGGTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT6770.12125836449870322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.11695969278826177No Hit
GTATCAACGCAGAGTACGGGCCGTGGCGGCGACGACCCATTCGAACGTCT6530.11695969278826177No Hit
GACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCC6530.11695969278826177No Hit
CTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGA6510.11660147014572496No Hit
TTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT6500.11642235882445658No Hit
ATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC6490.11624324750318818No Hit
TTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTAC6480.11606413618191978No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT6380.11427302296923585No Hit
ACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG6340.11355657768416226No Hit
TCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT6330.11337746636289386No Hit
TGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC6310.11301924372035708No Hit
TTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTA6310.11301924372035708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.1121236871140151No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT6250.1119445757927467No Hit
AACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTG6220.11140724182894153No Hit
TCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG6180.11069079654386793No Hit
CTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATC6070.1087205720099156No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG6070.1087205720099156No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGC6060.10854146068864721No Hit
GTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCA6050.10836234936737882No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA6000.10746679276103684No Hit
CGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGC5950.10657123615469488No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG5710.1022725644442534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3500.0103.57622570
AAGGGGG3050.075.2438169
AAAGGGG3100.068.0966768
GGTCAGA150.003275770563.48732416
CATAGTT251.3583424E-462.77746257
AAAAAGG3350.059.5598566
CATCTTT301.8764749E-458.80504267
CGACTAA302.0375826E-457.83347366
AAAAGGG3550.057.1485667
CTTTCGG552.3670006E-456.09544470
TCTTCTG3850.056.07313550
CTAATCA451.1320013E-555.43324769
CTTGAAA4050.055.22091357
CGTCTTC3950.053.65617448
TCGTATG3900.053.1078740
CTCGTAT2450.052.86176739
TTGCGAA200.006884269452.62591642
ATCTTTC353.3022405E-452.4471268
CGTATGC4050.052.4324541
TCTGCTT4150.052.32061453
Produced by FastQC (version 0.11.5)