FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933049
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAACCTAAATGGTCCGATATTCGG79560.8526883368397588No Hit
GTAATAATCAGCCTCATCTTCAGACTGGAGCCCACTGATGGCCAGGGAGG61830.6626661622272785No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58710.629227403919837No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGG45180.4842189424135281No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43930.47082200398907237No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37530.40222967925585895No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCATTTAGG36900.3954776222899334No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35900.38476007155036873No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTG34020.36461107615998734No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32900.352607419331675No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32530.3486419255580361No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31120.33353017901525No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30830.33042208930077627No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28080.30094882476697365No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCT27500.2947326453380262No Hit
GTATTACTTCCGATATTGGAGCTCCTTCCAGAGCAAGAGATGGTGACCGT27160.29108867808657424No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26060.2792993722730532No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25390.2721186132775449No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25230.2704038051592146No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25230.2704038051592146No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24800.2657952583412018No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24240.2597934299270456No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24100.2582929728235066No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22430.2403946630884337No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22420.2402874875810381No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22210.23803680192572954No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCAC21890.23460718568906885No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21890.23460718568906885No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20910.22410398596429557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20190.21638734943180907No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCATTTAGGT19340.20727743130317913No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19070.20438369260349673No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19040.20406216608130975No Hit
GATAAACACCGGCCCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAGTC18890.2024545334703751No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18840.20191865593339686No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18800.20148995390381427No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18380.19698858259319715No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17530.18787866446456725No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT17420.18669973388321515No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17220.18455622373530223No Hit
TTATTACTGTGCATCATGGGATGACAACCTAAATGGTCCGATATTCGGCG17160.18391317069092833No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17110.18337729315395013No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16490.17673241169542006No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16370.17544630560667232No Hit
CCTAAATGGTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA16360.17533913009927668No Hit
CCCCTGAGGGCCGGTGTTTATCATTATAGATGAGAAATTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATTGA16220.17383867299573763No Hit
GAAGTAATACTGTCAATTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA16150.1730884444439681No Hit
GGCCATCAGTGGGCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGCAT15890.1703018812516813No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15830.16965882820730746No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGATTCGGTC15610.16730096704460323No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15610.16730096704460323No Hit
GTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC15340.1644072283449208No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAAGATG15260.16354982428575562No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGAC15140.16226371819700786No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15010.16087043660086447No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14800.15861975094555592No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT14660.15711929384201687No Hit
GTCTGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAACCTAAATGGTCCGATATTCGGCGGAGGGAC14440.15476143267931264No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14310.15336815108316926No Hit
GTGTTTATCATTATAGATGAGAAATTTGGGGGCCGTTCCTGGGAGCTGCT14260.15283227354619103No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14150.15165334296483893No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14090.15101028992046506No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14060.1506887633982781No Hit
CAGTAATAATCAGCCTCATCTTCAGACTGGAGCCCACTGATGGCCAGGGA13900.14897395527994778No Hit
GCTTGGTCCCTCCGCCGAATATCGGACCATTTAGGTTGTCATCCCATGATGCACAGTAATAATCAGCCTCATCTT13810.148009375713387No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13720.14704479614682614No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13530.1450084615063089No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13260.14211472280662643No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13210.1415788452696482No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG13120.1406142657030874No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGCTCTGG13050.13986403715131787No Hit
TCTTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGATTCGGTCAGGGACC12920.1384707555551745No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12880.1380420535255919No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12690.13600571888507462No Hit
CCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT12520.13418373525934865No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12510.134076559751953No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12480.13375503322976606No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC12440.13332633120018347No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12310.1319330496040401No Hit
CCATTTAGGTTGTCATCCCATGATGCACAGTAATAATCAGCCTCATCTTC12230.1310756455448749No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12170.13043259250050104No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11840.12689580075644472No Hit
CTCTTGCTCTGGAAGGAGCTCCAATATCGGAAGTAATACTGTCAATTGGTACCAGCAGCTCCCAGGAACGGCCCC11830.1267886252490491No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11710.12550251916030133No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11670.12507381713071875No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11650.12485946611592746No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11450.12271595596801453No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT11450.12271595596801453No Hit
GGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC11140.11939351523874951No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11140.11939351523874951No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG11140.11939351523874951No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11090.11885763770177128No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11040.11832176016479305No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10820.11596389900208885No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG10810.1158567234946932No Hit
CCCAGGAACGGCCCCCAAATTTCTCATCTATAATGATAAACACCGGCCCTCAGGGGTCCCTGACCGAATCTCTGG10760.11532084595771497No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10750.11521367045031931No Hit
GTGCCAGACTTGGAGCCAGAGATTCGGTCAGGGACCCCTGAGGGCCGGTG10740.11510649494292369No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10580.11339168682459334No Hit
ACCTAAATGGTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA10470.11221275624324124No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA10460.1121055807358456No Hit
GTGCATCATGGGATGACAACCTAAATGGTCCGATATTCGGCGGAGGGACC10400.11146252769147172No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT10380.11124817667668044No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT10380.11124817667668044No Hit
GTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC10310.1104979481249109No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC10290.11028359711011962No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10080.10803291145481105No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10010.10728268290304153No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9770.10471047072554603No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9760.10460329521815039No Hit
GTTCCTGGGAGCTGCTGGTACCAATTGACAGTATTACTTCCGATATTGGA9740.1043889442033591No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9650.10342436463679829No Hit
GAGCAAGAGATGGTGACCGTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG9620.10310283811461134No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAAGATGAGGCTGAT9580.10267413608502876No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC9560.10245978507023748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAC200.005176966556.5605767
ACGTCAA200.00543402555.8722366
AGGGGGG2300.053.24065470
GTGGTAT2000.045.6222461
TCTCGTA1800.043.76328338
GGTATCA8550.043.4779971
TGGTATC2100.043.4427762
TCGTATG1950.042.3072440
CGTATGC2000.041.33067341
ATGCCGT2050.040.61548244
CTTATAC2000.040.553111
TCTCCGA2000.040.5465911
CCGTCTT2050.039.18622247
AACTGTA700.001444085538.8741370
ATGCGCA2100.038.72995829
ACGAGAC2100.038.62615222
ATCTCCG2100.038.61580310
CATGCGC2150.037.8109728
ACATGCG2150.037.7886427
CGAGACA2150.037.72989323
Produced by FastQC (version 0.11.5)