FastQCFastQC Report
Wed 8 May 2024
EGAF00004692146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933049
Total Bases68.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAACCTAAATGGTCCGATATTCGG80960.8676929078751492No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61690.6611657051237394No Hit
GTAATAATCAGCCTCATCTTCAGACTGGAGCCCACTGATGGCCAGGGAGG45190.4843261179209237No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCC43630.467606738767203No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42650.45710353904242973No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGG42560.4561389594758689No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40300.43191729480445296No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCATTTAGG38860.41648402173947996No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC38340.4109108953549064No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36540.39161930402369005No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG35900.38476007155036873No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCT35780.383473965461621No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32460.34789169700626654No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32250.345641011350958No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA31160.33395888104483257No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30690.3289216321972372No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA30120.3228126282756854No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA28480.3052358450627995No Hit
GTATTACTTCCGATATTGGAGCTCCTTCCAGAGCAAGAGATGGTGACCGT27910.29912684114124766No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG26750.28669448228335276No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26460.283586392568879No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCAC26210.28090700488398784No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26170.2804783028544053No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25120.2692248745778625No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24420.26172258906016727No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24170.25904320137527614No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA23740.25443465455726333No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23470.25154091585758087No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT23070.24725389556175506No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGAC22990.2463964915025899No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22710.2433955772955118No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT22570.2418951201919728No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA21880.2345000101816732No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21850.2341784836594863No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21680.2323565000337603No Hit
GAAGTAATACTGTCAATTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA21650.23203497351157337No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT21250.2277479532157475No Hit
GATAAACACCGGCCCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAGTC21160.2267833736491867No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20700.22185330030898698No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20310.21767345552055678No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACT20230.21681605146139163No Hit
GTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC20230.21681605146139163No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA19560.20963529246588336No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18600.19934644375590138No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC18390.1970957581005928No Hit
GGCCATCAGTGGGCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGCAT18190.19495224795267987No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC17730.19002217461248017No Hit
CCCCTGAGGGCCGGTGTTTATCATTATAGATGAGAAATTTGGGGGCCGTT17570.1883073664941498No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCGGACCATTTAGGT17550.18809301547935853No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17460.1871284359127977No Hit
GTGTTTATCATTATAGATGAGAAATTTGGGGGCCGTTCCTGGGAGCTGCT17390.1863782073610282No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAAGATG17370.18616385634623692No Hit
GTCTGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAACCTAA17290.18530645228707174No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTC17240.1847705747500935No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17090.18316294213915882No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCT17010.18230553807999367No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16650.17844721981375042No Hit
TTATTACTGTGCATCATGGGATGACAACCTAAATGGTCCGATATTCGGCG16510.17694676271021137No Hit
CCTAAATGGTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC16140.17298126893657248No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC16010.17158798734042907No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15970.17115928531084648No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGC15970.17115928531084648No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15790.16923012617772484No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCACCATCTCTTGCTCTGG15510.16622921197064677No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15060.1614063141378427No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGATTCGGTC15030.16108478761565578No Hit
GCTTGGTCCCTCCGCCGAATATCGGACCATTTAGGTTGTCATCCCATGAT15010.16087043660086447No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT14610.15658341630503864No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14570.15615471427545605No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA14340.1536896776053562No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC14170.15186769397963024No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14130.15143899195004762No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA14010.15015288586129988No Hit
GGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC14000.15004571035390427No Hit
CTCTTGCTCTGGAAGGAGCTCCAATATCGGAAGTAATACTGTCAATTGGT13790.14779502469859568No Hit
CCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAGCC13750.1473663226690131No Hit
CCCAGGAACGGCCCCCAAATTTCTCATCTATAATGATAAACACCGGCCCT13740.14725914716161745No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG13690.14672326962463922No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC13630.14608021658026535No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13620.14597304107286968No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13570.14543716353589148No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13530.1450084615063089No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTC13430.14393670643235243No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA13390.14350800440276984No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13250.14200754729923082No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13140.1408286167178787No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13060.13997121265871354No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12840.1376133514960093No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12780.13697029845163544No Hit
GTCACCATCTCTTGCTCTGGAAGGAGCTCCAATATCGGAAGTAATACTGT12640.1354698413480964No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG12340.13225457612622704No Hit
GTGCATCATGGGATGACAACCTAAATGGTCCGATATTCGGCGGAGGGACC12250.1312899965596662No Hit
GTTCCTGGGAGCTGCTGGTACCAATTGACAGTATTACTTCCGATATTGGA12170.13043259250050104No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG12150.13021824148570976No Hit
GTGCCAGACTTGGAGCCAGAGATTCGGTCAGGGACCCCTGAGGGCCGGTG12090.1295751884413359No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12030.128932135396962No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11950.12807473133779684No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGT11920.1277532048156099No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG11900.1275388538008186No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11780.12625274771207087No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAA11680.12518099263811439No Hit
GTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG11640.12475229060853181No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11640.12475229060853181No Hit
GTCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11630.12464511510113616No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG11530.12357336002717971No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCC11430.12250160495322325No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11310.12121549886447551No Hit
GCCCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAG11260.12067962132749728No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11240.12046527031270597No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA11070.11864328668698No Hit
ACCTAAATGGTCCGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT11030.11821458465739743No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTT10840.11617825001688015No Hit
ATGATAAACACCGGCCCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAG10830.11607107450948449No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCT10830.11607107450948449No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCA10740.11510649494292369No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGT10650.11414191537636287No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG10650.11414191537636287No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG10590.113498862331989No Hit
AGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACG10550.11307016030240641No Hit
CCATTTAGGTTGTCATCCCATGATGCACAGTAATAATCAGCCTCATCTTC10530.11285580928761513No Hit
GGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA10490.11242710725803255No Hit
CAGTAATAATCAGCCTCATCTTCAGACTGGAGCCCACTGATGGCCAGGGA10480.1123199317506369No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG10350.11092665015449349No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA10250.10985489508053704No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG10160.10889031551397621No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG10100.10824726246960235No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGA10090.1081400869622067No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAAGATGAGGCTGAT10080.10803291145481105No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC10010.10728268290304153No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC9990.10706833188825024No Hit
GAGCAAGAGATGGTGACCGTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG9940.10653245435127201No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG9780.10481764623294168No Hit
GTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGG9760.10460329521815039No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG9740.1043889442033591No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC9670.10363871565158957No Hit
GTGGGCTCCAGTCTGAAGATGAGGCTGATTATTACTGTGCATCATGGGAT9530.10213825854805053No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT9490.10170955651846794No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT9420.10095932796669843No Hit
CTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGT9410.10085215245930278No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAG9380.10053062593711586No Hit
GTCAGGGACCCCTGAGGGCCGGTGTTTATCATTATAGATGAGAAATTTGG9360.10031627492232455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)