FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843228
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAATACCGCTCACTTTCGGCGGCGGGACCAACGTGGAGATCAAACGA41680.49429098654219267No Hit
GTATTCTACCGTACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGGGTGAAGT37610.4460240883841618No Hit
GTATTACTGTCAGCAGTACGGTAGAATACCGCTCACTTTCGGCGGCGGGA35010.41519019766895787No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32580.38637236903897876No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGGGTGAAGTCTGTCCCAGACGCACTGCCA24640.29221041047024054No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22840.2708638707443301No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20970.24868718780685653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18250.2164301944432585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18020.21370258103383666No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17460.20706143534133117No Hit
GTGTATTACTGTCAGCAGTACGGTAGAATACCGCTCACTTTCGGCGGCGGGACCAACGTGGAGATCAAACGAA16980.20136902474775503No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16690.19792986001413615No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16360.1940163277310526No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16000.1897470197858705No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15470.18346164975546353No Hit
GTACATATATTATGCAGAGTCAATGAAGGGCCGATTCACCATCTCCAGAGACAATGACAAGAACTCTCTGTAT15450.1832244659807312No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15400.18263150654390034No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGCGTCTGGGACAGACTTCAC14900.1767019121755919No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14780.17527880952719785No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC14490.17183964479357897No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14280.1693492151588894No Hit
GTCCCCAGGCACCCTGTCTCTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG14110.16733315307366453No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13820.16389398834004565No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGGGTGAAGTCTGTCCCAGACGCACTGCCA13620.16152215059272224No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13530.1604548236064267No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12950.15357649413918895No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12910.15310212658972425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12800.1517976158286964No Hit
GTACGGTAGAATACCGCTCACTTTCGGCGGCGGGACCAACGTGGAGATCAAACGAACTGTGGCTGCACCAT12770.1514418401665979No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12420.14729112410878198No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12390.14693534844668346No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12360.14657957278458494No Hit
GACTTCACCCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTACGGTAGAATA12190.14456351069936008No Hit
ATATATGTACTGACTACCACTGCTAATGGATGAGACCCACTCCAGGCCCTTCCCTGGAGCCTGCCGGACCCAGTT11700.1387525082184178No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11330.13436460838586953No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11230.13317868951220785No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA10970.13009530044068746No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCCCGCCGCCGAAAGTG10940.12973952477858894No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10700.12689331948180088No Hit
GTTCAGTGGCAGTGCGTCTGGGACAGACTTCACCCTCACCATCACCAGAC10570.12535162494604069No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10240.12143809266295712No Hit
GGGTGAAGTCTGTCCCAGACGCACTGCCACTGAACCTGTCAGGGATGCCA10160.12048935756402777No Hit
ATAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA10080.1195406224650984No Hit
GTCTGGGACAGACTTCACCCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA9920.1176431522672397No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACGTTGGTCC9850.11681300905567653No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCAT9680.11479694697045163No Hit
CTACCGTACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGGGTGAAGTCTGTC9210.1092231282642417No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9180.1088673526021432No Hit
GTTTATTATTGTGCGAGAGACCCGGGCGATTTTTGGAGTGGTTATCCCTACTTCCAGCGCTGGGGCCAGGGCACC9160.10863016882741085No Hit
GTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGC9140.10839298505267853No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9100.10791861750321383No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9090.10780002561584767No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9060.10744424995374915No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9020.10696988240428448No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8880.1053095959811581No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8510.10092169614860987No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8480.10056592048651135No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8470.1004473285991452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT350.005109858256.7427170
GGTATCA6850.043.3425751
GTATCAA19050.038.6941071
TACGTAT700.001650994837.82847270
TAGTCAC852.9867806E-936.50419238
AATAGTC853.0613592E-936.41243436
TATCAAC21200.034.9060362
AATGGTA5150.034.70670370
AGACGTT400.002535724334.6654542
TCAACGC22300.033.4801254
ATCAACG22250.033.4001583
CAACGCA22700.032.892125
AACGCAG23000.032.4630936
GTGGTGA5000.031.77591770
TCGCTAG450.00410916131.41257358
TTCGCTA450.004127749231.38362357
ACGCAGA24350.030.8033057
GTACGTA703.6192566E-430.80217269
CGCAGAG24350.030.5232758
GAGTTTA3150.030.334271
Produced by FastQC (version 0.11.5)