FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004692151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120216
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT15761.3109735808877354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.39345844147201703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.31942503493711316No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.31526585479470287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.27284221734211755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.25370998868703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.24705530045917348No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC2930.24372795634524524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.23540959606042455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.22376389166167565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.2171092034338191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2400.19964064683569574No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.18549943435150062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.17718107406668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.17718107406668No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.1671990417248952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.16636720569641314No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.16387169761096693No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATGTCGTATGCCGT1960.16303986158248485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.1622080255540028No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.15888068144007453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.15888068144007453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.1455713049843615No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.13808478072802288No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.13725294469954083No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.13642110867105878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.1305982564716843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.12976642044320225No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG1560.12976642044320225No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.12477540427230985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.1231117322153457No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG1450.12061622412989952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1430.11895255207293538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.11645704398748918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.11562520795900713No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.113961535902043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1370.113961535902043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.11146602781659679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.10730684767418647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.10314766753177612No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1220.101483995474812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1220.101483995474812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.10065215944632994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)